Expression and function of TRAF-3 splice-variant isoforms in human lymphoma cell lines

Christopher Gamper, Coral O. Omene, Winfried G. Van Eyndhoven, Grace D. Glassman, Seth Lederman

Research output: Contribution to journalArticle

Abstract

TRAF-3 gene products are signaling adaptor molecules required for lymphocytes to mediate T-dependent antibody responses in vivo. Previous work identified 8 splice-variant TRAF-3 mRNA species by RT-PCR that have the potential to encode novel isoforms, seven of which induce NF-κB activation when over-expressed in 293 cells. Here, their expression was characterized by RNAse protection assay, which showed the T cell line Jurkat D1.1 and the B cell lines BJAB, Daudi, and Raji each expressed mRNA encoding TRAF-3 splice-variants in approximately the same rank order (from highest to lowest); TRAF-3 Δ103aa, Δ83aa, full-length, Δ25aa, Δ52aa, Δ56aa, Δ27aa, and Δ221aa mRNA. The TRAF-3 Δ130aa mRNA was not detectable in any of the cell lines examined. The functional effect of over-expressing each TRAF-3 splice-variant on NF-κB activation was studied in the TRAF-5-responsive B cell line, BJAB. Of the seven TRAF-3 splice-variant isoforms that induce NF-κB activation in 293 cells, only TRAF-3 Δ27aa, Δ103aa, or Δ130aa induce NF-κB activation in BJAB cells. Together, these data indicate that a number of TRAF-3 splice-variant mRNAs are expressed and function in B and T lymphoma lines, which suggests that certain TRAF-3 splice-variant isoforms may participate in mediating the known functions of the TRAF-3 gene in lymphocytes.

Original languageEnglish (US)
Pages (from-to)1167-1177
Number of pages11
JournalHuman Immunology
Volume62
Issue number10
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Keywords

  • Cell surface molecules
  • Human
  • Molecular biology
  • Signal transduction
  • T cell-B cell collaboration

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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