Expression and function of a recombinant PDGF B gene in porcine arteries

Vincent J. Pompili, David Gordon, Hong San, Zhiyong Yang, David W M Muller, Gary J. Nabel, Elizabeth G. Nabel

Research output: Contribution to journalArticlepeer-review

Abstract

Platelet derived growth factor (PDGF) B is a mitogen and chemoattractant for smooth muscle cells in vitro, and expression of a recombinant PDGF B gene in porcine arteries stimulates intimal thickening. To define the mechanisms by which PDGF B gene expression induces intimal thickening in vivo, we examined its effects on smooth muscle cell proliferation and migration, extracellular matrix synthesis, and inflammatory cell infiltration in intimal lesions of pig arteries after direct gene transfer of a recombinant PDGF B gone. PDGF B gent expression was associated with rapid formation of an intima, including 3- to 10-fold increases in intimal thickness and intima- to-media area ratio 4 to 21 days after gene transfer compared with control transfected arteries. Intimal smooth muscle cell proliferation was detected at 2 days, peaked at 7 days (P

Original languageEnglish (US)
Pages (from-to)2254-2264
Number of pages11
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume15
Issue number12
StatePublished - Dec 1995
Externally publishedYes

Keywords

  • cell proliferation
  • gene expression
  • gene transfer
  • neointima
  • PDGF B gene

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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