Abstract
Platelet derived growth factor (PDGF) B is a mitogen and chemoattractant for smooth muscle cells in vitro, and expression of a recombinant PDGF B gene in porcine arteries stimulates intimal thickening. To define the mechanisms by which PDGF B gene expression induces intimal thickening in vivo, we examined its effects on smooth muscle cell proliferation and migration, extracellular matrix synthesis, and inflammatory cell infiltration in intimal lesions of pig arteries after direct gene transfer of a recombinant PDGF B gone. PDGF B gent expression was associated with rapid formation of an intima, including 3- to 10-fold increases in intimal thickness and intima- to-media area ratio 4 to 21 days after gene transfer compared with control transfected arteries. Intimal smooth muscle cell proliferation was detected at 2 days, peaked at 7 days (P
Original language | English (US) |
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Pages (from-to) | 2254-2264 |
Number of pages | 11 |
Journal | Arteriosclerosis, Thrombosis, and Vascular Biology |
Volume | 15 |
Issue number | 12 |
State | Published - Dec 1995 |
Externally published | Yes |
Keywords
- cell proliferation
- gene expression
- gene transfer
- neointima
- PDGF B gene
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine