Expression and distribution of amyloid precursor protein-like protein-2 in Alzheimer's disease and in normal brain

Barbara J. Crain, Weidong Hu, Chun I. Sze, Hilda H. Slunt, Edward H. Koo, Donald L. Price, Gopal Thinakaran, Sangram S. Sisodia

Research output: Contribution to journalArticle

Abstract

Amyloid precursor-like protein-2 (APLP-2) belongs to a family of homologous amyloid precursor-like proteins. In the present study we report on the expression and distribution of APLP-2 in fetal and adult human brain and in brains of patients with Alzheimer's disease. We demonstrate that APLP-2 mRNAs encoding isoforms predicted to undergo post-translational modification by chondroitin sulfate glycosaminoglycans are elevated in fetal and aging brains relative to the brains of young adults. Immunocytochemical labeling with APLP-2-specific antibodies demonstrates APLP-2 immunoreactivity in cytoplasmic compartments in neurons and astrocytes, in large part overlapping the distribution of the amyloid precursor protein. In Alzheimer's disease brain, APLP-2 antibodies also label a subset of neuritic plaques. APLP-2 immunoreactivity is particularly conspicuous in large dystrophic neurites that also label with antibodies specific for APP and chromogranin A. In view of the age-dependent increase in levels of chondroitin sulfate glycosaminogycan-modified forms of APLP-2 in aging brain and the accumulation of APLP-2 in dystrophic presynaptic elements, we suggest that APLP-2 may play roles in neuronalsprouting or in the aggregation, deposition, and/or persistence of β-amyloid deposits.

Original languageEnglish (US)
Pages (from-to)1087-1095
Number of pages9
JournalAmerican Journal of Pathology
Volume149
Issue number4
StatePublished - 1996

Fingerprint

Amyloid beta-Protein Precursor
Brain
Proteins
Amyloid Plaques
Alzheimer disease type 2
Antibodies
Alzheimer Disease
RNA Isoforms
Chromogranin A
Chondroitin Sulfates
Neurites
Post Translational Protein Processing
Astrocytes
Young Adult

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Crain, B. J., Hu, W., Sze, C. I., Slunt, H. H., Koo, E. H., Price, D. L., ... Sisodia, S. S. (1996). Expression and distribution of amyloid precursor protein-like protein-2 in Alzheimer's disease and in normal brain. American Journal of Pathology, 149(4), 1087-1095.

Expression and distribution of amyloid precursor protein-like protein-2 in Alzheimer's disease and in normal brain. / Crain, Barbara J.; Hu, Weidong; Sze, Chun I.; Slunt, Hilda H.; Koo, Edward H.; Price, Donald L.; Thinakaran, Gopal; Sisodia, Sangram S.

In: American Journal of Pathology, Vol. 149, No. 4, 1996, p. 1087-1095.

Research output: Contribution to journalArticle

Crain, BJ, Hu, W, Sze, CI, Slunt, HH, Koo, EH, Price, DL, Thinakaran, G & Sisodia, SS 1996, 'Expression and distribution of amyloid precursor protein-like protein-2 in Alzheimer's disease and in normal brain', American Journal of Pathology, vol. 149, no. 4, pp. 1087-1095.
Crain, Barbara J. ; Hu, Weidong ; Sze, Chun I. ; Slunt, Hilda H. ; Koo, Edward H. ; Price, Donald L. ; Thinakaran, Gopal ; Sisodia, Sangram S. / Expression and distribution of amyloid precursor protein-like protein-2 in Alzheimer's disease and in normal brain. In: American Journal of Pathology. 1996 ; Vol. 149, No. 4. pp. 1087-1095.
@article{0ba7ca1f57464e70a966ab7425bba0a5,
title = "Expression and distribution of amyloid precursor protein-like protein-2 in Alzheimer's disease and in normal brain",
abstract = "Amyloid precursor-like protein-2 (APLP-2) belongs to a family of homologous amyloid precursor-like proteins. In the present study we report on the expression and distribution of APLP-2 in fetal and adult human brain and in brains of patients with Alzheimer's disease. We demonstrate that APLP-2 mRNAs encoding isoforms predicted to undergo post-translational modification by chondroitin sulfate glycosaminoglycans are elevated in fetal and aging brains relative to the brains of young adults. Immunocytochemical labeling with APLP-2-specific antibodies demonstrates APLP-2 immunoreactivity in cytoplasmic compartments in neurons and astrocytes, in large part overlapping the distribution of the amyloid precursor protein. In Alzheimer's disease brain, APLP-2 antibodies also label a subset of neuritic plaques. APLP-2 immunoreactivity is particularly conspicuous in large dystrophic neurites that also label with antibodies specific for APP and chromogranin A. In view of the age-dependent increase in levels of chondroitin sulfate glycosaminogycan-modified forms of APLP-2 in aging brain and the accumulation of APLP-2 in dystrophic presynaptic elements, we suggest that APLP-2 may play roles in neuronalsprouting or in the aggregation, deposition, and/or persistence of β-amyloid deposits.",
author = "Crain, {Barbara J.} and Weidong Hu and Sze, {Chun I.} and Slunt, {Hilda H.} and Koo, {Edward H.} and Price, {Donald L.} and Gopal Thinakaran and Sisodia, {Sangram S.}",
year = "1996",
language = "English (US)",
volume = "149",
pages = "1087--1095",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Expression and distribution of amyloid precursor protein-like protein-2 in Alzheimer's disease and in normal brain

AU - Crain, Barbara J.

AU - Hu, Weidong

AU - Sze, Chun I.

AU - Slunt, Hilda H.

AU - Koo, Edward H.

AU - Price, Donald L.

AU - Thinakaran, Gopal

AU - Sisodia, Sangram S.

PY - 1996

Y1 - 1996

N2 - Amyloid precursor-like protein-2 (APLP-2) belongs to a family of homologous amyloid precursor-like proteins. In the present study we report on the expression and distribution of APLP-2 in fetal and adult human brain and in brains of patients with Alzheimer's disease. We demonstrate that APLP-2 mRNAs encoding isoforms predicted to undergo post-translational modification by chondroitin sulfate glycosaminoglycans are elevated in fetal and aging brains relative to the brains of young adults. Immunocytochemical labeling with APLP-2-specific antibodies demonstrates APLP-2 immunoreactivity in cytoplasmic compartments in neurons and astrocytes, in large part overlapping the distribution of the amyloid precursor protein. In Alzheimer's disease brain, APLP-2 antibodies also label a subset of neuritic plaques. APLP-2 immunoreactivity is particularly conspicuous in large dystrophic neurites that also label with antibodies specific for APP and chromogranin A. In view of the age-dependent increase in levels of chondroitin sulfate glycosaminogycan-modified forms of APLP-2 in aging brain and the accumulation of APLP-2 in dystrophic presynaptic elements, we suggest that APLP-2 may play roles in neuronalsprouting or in the aggregation, deposition, and/or persistence of β-amyloid deposits.

AB - Amyloid precursor-like protein-2 (APLP-2) belongs to a family of homologous amyloid precursor-like proteins. In the present study we report on the expression and distribution of APLP-2 in fetal and adult human brain and in brains of patients with Alzheimer's disease. We demonstrate that APLP-2 mRNAs encoding isoforms predicted to undergo post-translational modification by chondroitin sulfate glycosaminoglycans are elevated in fetal and aging brains relative to the brains of young adults. Immunocytochemical labeling with APLP-2-specific antibodies demonstrates APLP-2 immunoreactivity in cytoplasmic compartments in neurons and astrocytes, in large part overlapping the distribution of the amyloid precursor protein. In Alzheimer's disease brain, APLP-2 antibodies also label a subset of neuritic plaques. APLP-2 immunoreactivity is particularly conspicuous in large dystrophic neurites that also label with antibodies specific for APP and chromogranin A. In view of the age-dependent increase in levels of chondroitin sulfate glycosaminogycan-modified forms of APLP-2 in aging brain and the accumulation of APLP-2 in dystrophic presynaptic elements, we suggest that APLP-2 may play roles in neuronalsprouting or in the aggregation, deposition, and/or persistence of β-amyloid deposits.

UR - http://www.scopus.com/inward/record.url?scp=0029794266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029794266&partnerID=8YFLogxK

M3 - Article

C2 - 8863657

AN - SCOPUS:0029794266

VL - 149

SP - 1087

EP - 1095

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 4

ER -