Expressed mu opiate receptor couples to adenylate cyclase and phosphatidyl inositol turnover

Peter S. Johnson; Jia Bei Wang; Wen Fei Wang; George R. Uhl

Expressed mu opiate receptor couples to adenylate cyclase and phosphatidyl inositol turnover

Peter S. Johnson, Jia Bei Wang, Wen Fei Wang, George R. Uhl

School of Medicine

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

The recently cloned rat mu opiate receptor cDNA has been expressed in COS and Chinese hamster ovary (CHO) cells to examine the coupling of this receptor to G-protein linked second messenger systems and examine possible coupling to multiple second messenger systems. Morphine (1µM) reduced both forskolin-stimulated cAMP levels and IP3 levels by 20 ± 5 and 34 ± 8% respectively in COS and CHO cell cultures expressing the cloned rat mu receptor cDNA. Both effects could be blocked by naloxone and Gpp(NH)p. These results represent the first clear representation of the second messenger system promiscuity possible with a single cloned opiate receptor.

Original language	English (US)
Pages (from-to)	507-509
Number of pages	3
Journal	NeuroReport
Volume	5
Issue number	4
State	Published - 1994

Keywords

Analgesia
Drug abuse
G-protein
Morphine
Pain

ASJC Scopus subject areas

General Neuroscience

Cite this

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abstract = "The recently cloned rat mu opiate receptor cDNA has been expressed in COS and Chinese hamster ovary (CHO) cells to examine the coupling of this receptor to G-protein linked second messenger systems and examine possible coupling to multiple second messenger systems. Morphine (1µM) reduced both forskolin-stimulated cAMP levels and IP3 levels by 20 ± 5 and 34 ± 8% respectively in COS and CHO cell cultures expressing the cloned rat mu receptor cDNA. Both effects could be blocked by naloxone and Gpp(NH)p. These results represent the first clear representation of the second messenger system promiscuity possible with a single cloned opiate receptor.",

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T1 - Expressed mu opiate receptor couples to adenylate cyclase and phosphatidyl inositol turnover

AU - Johnson, Peter S.

AU - Wang, Jia Bei

AU - Wang, Wen Fei

AU - Uhl, George R.

PY - 1994

Y1 - 1994

N2 - The recently cloned rat mu opiate receptor cDNA has been expressed in COS and Chinese hamster ovary (CHO) cells to examine the coupling of this receptor to G-protein linked second messenger systems and examine possible coupling to multiple second messenger systems. Morphine (1µM) reduced both forskolin-stimulated cAMP levels and IP3 levels by 20 ± 5 and 34 ± 8% respectively in COS and CHO cell cultures expressing the cloned rat mu receptor cDNA. Both effects could be blocked by naloxone and Gpp(NH)p. These results represent the first clear representation of the second messenger system promiscuity possible with a single cloned opiate receptor.

AB - The recently cloned rat mu opiate receptor cDNA has been expressed in COS and Chinese hamster ovary (CHO) cells to examine the coupling of this receptor to G-protein linked second messenger systems and examine possible coupling to multiple second messenger systems. Morphine (1µM) reduced both forskolin-stimulated cAMP levels and IP3 levels by 20 ± 5 and 34 ± 8% respectively in COS and CHO cell cultures expressing the cloned rat mu receptor cDNA. Both effects could be blocked by naloxone and Gpp(NH)p. These results represent the first clear representation of the second messenger system promiscuity possible with a single cloned opiate receptor.

KW - Analgesia

KW - Drug abuse

KW - G-protein

KW - Morphine

KW - Pain

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ER -

Expressed mu opiate receptor couples to adenylate cyclase and phosphatidyl inositol turnover

Abstract

Keywords

ASJC Scopus subject areas

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