Exposure to 1 ppm ozone attenuates the immediate antigenic response of canine peripheral airways

Steven R. Kleeberger, John Kolbe, Claudia Turner, Ernst W. Spannhake

Research output: Contribution to journalArticle

Abstract

The effect of oxidant exposure on the immediate airway response to immunologic challenge is controversial. We investigated the response of canine peripheral airways to antigen aerosol, 1-3 h and 24 h after a 5-min exposure to 1 ppm ozone. In dogs that were natively sensitive to Ascaris suum antigen, resistance to flow through the collateral system (Rcs) was measured using the wedged bronchoscope technique. In eight dogs, four sublobar segments of each lung were wedged: Two were exposed to ozone for 5 min and two (control) received air with 5% C02. Ozone caused a mean (±SE) increase in Rcsof 75 ± 15%, which returned to baseline after 1-3 h. The increase in Rcselicited by subsequent administration of antigen aerosol (25 μl, 0.27 mg proteinlml) to the ozone-exposed segments (312.0 ± 70.6%) was attenuated by 22% compared to controls (398.9 ± 83.0%; p <.05). In another series of experiments (n = 5), segments were exposed to ozone or air and challenged with antigen 24 h laterand a significant attenuation (38%) of the antigen-induced increase in Rcs was detected compared to controls (178.5 ± 57.9 vs. 289.0 ± 62.2; p <.05). Cellular influx of polymorphonuclear leukocytes (PMNs) was not detected by bronchoalveolar lavage (BAL) 1-3 h after ozone, but was found after24 h (19.8 vs. 4.7%; p <.01). A significant increase in PMNs was detected in exposed subepithelial tissues 1-3 h after ozone compared to unexposed tissues. Tissue PMNs were not significantly different from unexposed tissues after 24 h, but a shift toward degranulation of mast cells was detected in ozone-exposed tissues at this time. These data suggest that the Rcsresponse to antigen is attenuated 1-3 h and 24 h after acute (5 min) exposure to 1 ppm ozone, and this effect occurs independently of PMNs in the airways.

Original languageEnglish (US)
Pages (from-to)349-362
Number of pages14
JournalJournal of Toxicology and Environmental Health
Volume28
Issue number3
DOIs
StatePublished - Nov 1 1989

ASJC Scopus subject areas

  • Toxicology
  • Pollution

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