Abstract
We previously demonstrated that small-particle (0.5 – 3.0 µm) aerosol infection of rhesus monkeys (Macaca mulatta) with cowpox virus (CPXV)-Brighton Red (BR) results in fulminant respiratory tract disease characterized by severe lung parenchymal pathology but only limited systemic virus dissemination and limited classic epidermal pox-like lesion development (Johnson et al., 2015). Based on these results, and to further develop CPXV as an improved model of human smallpox, we evaluated a novel large-particle aerosol (7.0 – 9.0 µm) exposure of rhesus monkeys to CPXV- BR and monitored for respiratory tract disease by serial computed tomography (CT). As expected, the upper respiratory tract and large airways were the major sites of virus-induced pathology following large-particle aerosol exposure. Large-particle aerosol CPXV exposure of rhesus macaques resulted in severe upper airway and large airway pathology with limited systemic dissemination.
Original language | English (US) |
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Article number | 000501 |
Pages (from-to) | 1942-1954 |
Number of pages | 13 |
Journal | Journal of General Virology |
Volume | 97 |
Issue number | 8 |
DOIs | |
State | Published - Aug 2016 |
Keywords
- Aerosol
- Large-particle aerosol
- Nonhuman primate model
- Orthopoxvirus
- Smallpox
- Variola virus
ASJC Scopus subject areas
- Virology