Exploring functional in vivo consequences of the selective genetic ablation of mTOR signaling in T helper lymphocytes

Greg M. Delgoffe, Jonathan D. Powell

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The mammalian Target of Rapamycin (mTOR) defines a crucial link between nutrient sensing and immune function. In CD4+ T cells, mTOR has been shown to play a critical role in regulating effector and regulatory T cell differentiation as well as the decision between full activation versus the induction of anergy. In this chapter, we describe how our group has employed the Cre-lox technology to genetically delete components of the mTOR signaling complex in T cells. This has enabled us to specifically interrogate mTOR function in T cells both in vitro and in vivo. We also describe techniques used to assay immune function and signaling in mTOR-deficient T cells at the single-cell level.

Original languageEnglish (US)
Title of host publicationmTOR
Subtitle of host publicationMethods and Protocols
EditorsThomas Weichhart
Pages317-327
Number of pages11
DOIs
StatePublished - Jan 2 2012

Publication series

NameMethods in Molecular Biology
Volume821
ISSN (Print)1064-3745

Keywords

  • CD4
  • T cells
  • mTOR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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