@inbook{35017006b9eb410abe1f0ea17fd08476,
title = "Exploring functional in vivo consequences of the selective genetic ablation of mTOR signaling in T helper lymphocytes",
abstract = "The mammalian Target of Rapamycin (mTOR) defines a crucial link between nutrient sensing and immune function. In CD4+ T cells, mTOR has been shown to play a critical role in regulating effector and regulatory T cell differentiation as well as the decision between full activation versus the induction of anergy. In this chapter, we describe how our group has employed the Cre-lox technology to genetically delete components of the mTOR signaling complex in T cells. This has enabled us to specifically interrogate mTOR function in T cells both in vitro and in vivo. We also describe techniques used to assay immune function and signaling in mTOR-deficient T cells at the single-cell level.",
keywords = "CD4, T cells, mTOR",
author = "Delgoffe, {Greg M.} and Powell, {Jonathan D.}",
year = "2012",
doi = "10.1007/978-1-61779-430-8_20",
language = "English (US)",
isbn = "9781617794292",
series = "Methods in Molecular Biology",
pages = "317--327",
editor = "Thomas Weichhart",
booktitle = "mTOR",
}