Explant organ culture: A review

James H. Resau, Kosaku Sakamoto, John R. Cottrell, Eric A. Hudson, Stephen J. Meltzer

Research output: Contribution to journalReview articlepeer-review

38 Scopus citations


Organ explant culture models offer several significant advantages for studies of patho-physiologic mechanisms like cell injury, secretion, differentiation and structure development. Organs or small explants/slices can be removed in vivo and maintained in vitro for extended periods of time if careful attention is paid to the media composition, substrate selection, and atmosphere. In the case of human tissues obtained from autopsy or surgery, additional attention must be paid to the postmortem interval, temperature, hydration, and cause of death. Explant organ culture has been effectively utilized to establish outgrowth cell cultures and characterize the histiotypic relationships between the various cell types within an organ or tissue.

Original languageEnglish (US)
Pages (from-to)137-149
Number of pages13
Issue number3
StatePublished - Nov 1991
Externally publishedYes


  • Organ culture
  • colon
  • epithelium
  • human

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology


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