Explanatory chapter: Troubleshooting protein expression: What to do when the protein is not soluble

Krisna C. Duong-Ly, Sandra B. Gabelli

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Production of soluble protein remains a bottleneck in the biochemistry and structural biology fields. Unfortunately, there is no 'magic bullet' that solves all solubility problems. The following is a protocol to test whether a protein expressed recombinantly is soluble, and possible strategies to circumvent insolubility issues.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part C
PublisherAcademic Press Inc.
Pages231-247
Number of pages17
ISBN (Print)9780124201194
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume541
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Cell lysis
  • Differential scanning fluorimetry (DSF)
  • IPTG
  • Immunoblotting
  • SDS-PAGE
  • Small ubiquitin-like modifier (SUMO)
  • Troubleshooting protein expression

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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