@inbook{03ed41a123fc48759476715fa162eb3d,
title = "Explanatory chapter: Troubleshooting protein expression: What to do when the protein is not soluble",
abstract = "Production of soluble protein remains a bottleneck in the biochemistry and structural biology fields. Unfortunately, there is no 'magic bullet' that solves all solubility problems. The following is a protocol to test whether a protein expressed recombinantly is soluble, and possible strategies to circumvent insolubility issues.",
keywords = "Cell lysis, Differential scanning fluorimetry (DSF), IPTG, Immunoblotting, SDS-PAGE, Small ubiquitin-like modifier (SUMO), Troubleshooting protein expression",
author = "Duong-Ly, {Krisna C.} and Gabelli, {Sandra B.}",
year = "2014",
doi = "10.1016/B978-0-12-420119-4.00018-5",
language = "English (US)",
isbn = "9780124201194",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "231--247",
booktitle = "Laboratory Methods in Enzymology",
}