Explanatory chapter: Next generation sequencing

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Technological breakthroughs in sequencing technologies have driven the advancement of molecular biology and molecular genetics research. The advent of high-throughput Sanger sequencing (for information on the method, see Sanger Dideoxy Sequencing of DNA) in the mid- to late-1990s made possible the accelerated completion of the human genome project, which has since revolutionized the pace of discovery in biomedical research. Similarly, the advent of next generation sequencing is poised to revolutionize biomedical research and usher a new era of individualized, rational medicine. The term next generation sequencing refers to technologies that have enabled the massively parallel analysis of DNA sequence facilitated through the convergence of advancements in molecular biology, nucleic acid chemistry and biochemistry, computational biology, and electrical and mechanical engineering. The current next generation sequencing technologies are capable of sequencing tens to hundreds of millions of DNA templates simultaneously and generate > 4 gigabases of sequence in a single day. These technologies have largely started to replace high-throughput Sanger sequencing for large-scale genomic projects, and have created significant enthusiasm for the advent of a new era of individualized medicine.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
Pages201-208
Number of pages8
Volume529
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume529
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Technology
Molecular Biology
Precision Medicine
Molecular biology
DNA Sequence Analysis
Medicine
Biomedical Research
Throughput
Human Genome Project
Genetic Research
Biochemistry
DNA sequences
DNA
Electrical engineering
Mechanical engineering
Computational Biology
Nucleic Acids
Genes
Genetics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Yegnasubramanian, S. (2013). Explanatory chapter: Next generation sequencing. In Methods in Enzymology (Vol. 529, pp. 201-208). (Methods in Enzymology; Vol. 529). https://doi.org/10.1016/B978-0-12-418687-3.00016-1

Explanatory chapter : Next generation sequencing. / Yegnasubramanian, S.

Methods in Enzymology. Vol. 529 2013. p. 201-208 (Methods in Enzymology; Vol. 529).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yegnasubramanian, S 2013, Explanatory chapter: Next generation sequencing. in Methods in Enzymology. vol. 529, Methods in Enzymology, vol. 529, pp. 201-208. https://doi.org/10.1016/B978-0-12-418687-3.00016-1
Yegnasubramanian S. Explanatory chapter: Next generation sequencing. In Methods in Enzymology. Vol. 529. 2013. p. 201-208. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-418687-3.00016-1
Yegnasubramanian, S. / Explanatory chapter : Next generation sequencing. Methods in Enzymology. Vol. 529 2013. pp. 201-208 (Methods in Enzymology).
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