Experimental model of obstructive, chronic pancreatitis in pigs

D. Boerma, I. H. Straatsburg, G. J A Offerhaus, D. J. Gouma, T. M. Van Gulik

Research output: Contribution to journalArticle

Abstract

Background: We aimed to develop a reproducible, experimental model of obstructive pancreatitis for future analysis of surgical interventions, and characterized this model using functional, histological and biochemical parameters. Animals and Methods: In 10 female pigs the pancreatic duct (PD) was ligated. After 4, 6 or 8 weeks the animals were sacrificed. Before and after ligation, glucose tolerance and intraductal pressure were measured, and pancreatic juice was collected after stimulation with cholecystokinin and secretin. Amylase and lipase activities were analyzed in plasma and juice. Pancreatic tissue was collected for histochemical analysis. Results: Within 4 weeks of ligation, the pancreas appeared atrophic. Intraductal pressure had risen significantly. Acinar-to-ductal metaplasia was accompanied by strong proliferation of stellate cells and increased collagen deposition. Islets of Langerhans appeared smaller and more numerous. Blood amylase and lipase levels were normal and glucose tolerance was unaffected. Pancreatic juice volume and amylase and lipase activities were significantly lower. Conclusion: Ligation of the PD in pigs resulted in a marked fibrosing obstructive pancreatitis within 4 weeks, similar to human chronic pancreatitis in regard to clinical, functional, histological and biochemical parameters.

Original languageEnglish (US)
Pages (from-to)520-526
Number of pages7
JournalDigestive Surgery
Volume20
Issue number6
DOIs
StatePublished - 2003
Externally publishedYes

Fingerprint

Chronic Pancreatitis
Amylases
Lipase
Ligation
Pancreatic Juice
Theoretical Models
Swine
Pancreatic Ducts
Pancreatitis
Pressure
Glucose
Secretin
Cholecystokinin
Metaplasia
Islets of Langerhans
Pancreas
Collagen
Cell Proliferation

Keywords

  • Animal model
  • Chronic inflammation
  • Chronic pancreatitis
  • Fibrosis
  • Stellate cells

ASJC Scopus subject areas

  • Surgery

Cite this

Boerma, D., Straatsburg, I. H., Offerhaus, G. J. A., Gouma, D. J., & Van Gulik, T. M. (2003). Experimental model of obstructive, chronic pancreatitis in pigs. Digestive Surgery, 20(6), 520-526. https://doi.org/10.1159/000073688

Experimental model of obstructive, chronic pancreatitis in pigs. / Boerma, D.; Straatsburg, I. H.; Offerhaus, G. J A; Gouma, D. J.; Van Gulik, T. M.

In: Digestive Surgery, Vol. 20, No. 6, 2003, p. 520-526.

Research output: Contribution to journalArticle

Boerma, D, Straatsburg, IH, Offerhaus, GJA, Gouma, DJ & Van Gulik, TM 2003, 'Experimental model of obstructive, chronic pancreatitis in pigs', Digestive Surgery, vol. 20, no. 6, pp. 520-526. https://doi.org/10.1159/000073688
Boerma D, Straatsburg IH, Offerhaus GJA, Gouma DJ, Van Gulik TM. Experimental model of obstructive, chronic pancreatitis in pigs. Digestive Surgery. 2003;20(6):520-526. https://doi.org/10.1159/000073688
Boerma, D. ; Straatsburg, I. H. ; Offerhaus, G. J A ; Gouma, D. J. ; Van Gulik, T. M. / Experimental model of obstructive, chronic pancreatitis in pigs. In: Digestive Surgery. 2003 ; Vol. 20, No. 6. pp. 520-526.
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