Experimental model of immune-mediated hearing loss using cross-species immunization

Charles R. Orozco, John K. Niparko, Bruce C. Richardson, David F. Dolan, Martin U. Ptok, Richard A. Altschuler

Research output: Contribution to journalArticle

Abstract

The presence of immune-mediated hearing loss was investigated in an animal model. Eight guinea pigs and four mice underwent immunizations with a preparation of chick or guinea pig cochlear tissue and Freund’s adjuvant. Hearing thresholds were monitored by auditory brainstem response (ABR) testing over a 5-week period after immunization. The serum and temporal bones of test and control animals were then examined using an enzyme-linked immunosorbent assay (ELISA), immunocytochemical, and histological techniques. Hearing loss of 20 dB or greater occurred in eight animals. ELISA demonstrated antibodies to cochlear antigens in the sera of all test animals. Immunocytochemistry revealed immunostaining of hair cell stereocilia in the organ of Corti and saccule. Endolymphatic hydrops, and organ of Corti degeneration was observed in the temporal bones of three animals. This study provides evidence to suggest that cross species immunization with cochlear antigens might produce a humoral response that can be associated with inner ear pathologic change and sensorineural hearing loss.

Original languageEnglish (US)
Pages (from-to)941-947
Number of pages7
JournalLaryngoscope
Volume100
Issue number9
StatePublished - 1990
Externally publishedYes

Fingerprint

Hearing Loss
Immunization
Cochlea
Theoretical Models
Organ of Corti
Temporal Bone
Guinea Pigs
Enzyme-Linked Immunosorbent Assay
Endolymphatic Hydrops
Stereocilia
Saccule and Utricle
Histological Techniques
Antigens
Brain Stem Auditory Evoked Potentials
Freund's Adjuvant
Sensorineural Hearing Loss
Inner Ear
Serum
Hearing
Animal Models

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Orozco, C. R., Niparko, J. K., Richardson, B. C., Dolan, D. F., Ptok, M. U., & Altschuler, R. A. (1990). Experimental model of immune-mediated hearing loss using cross-species immunization. Laryngoscope, 100(9), 941-947.

Experimental model of immune-mediated hearing loss using cross-species immunization. / Orozco, Charles R.; Niparko, John K.; Richardson, Bruce C.; Dolan, David F.; Ptok, Martin U.; Altschuler, Richard A.

In: Laryngoscope, Vol. 100, No. 9, 1990, p. 941-947.

Research output: Contribution to journalArticle

Orozco, CR, Niparko, JK, Richardson, BC, Dolan, DF, Ptok, MU & Altschuler, RA 1990, 'Experimental model of immune-mediated hearing loss using cross-species immunization', Laryngoscope, vol. 100, no. 9, pp. 941-947.
Orozco CR, Niparko JK, Richardson BC, Dolan DF, Ptok MU, Altschuler RA. Experimental model of immune-mediated hearing loss using cross-species immunization. Laryngoscope. 1990;100(9):941-947.
Orozco, Charles R. ; Niparko, John K. ; Richardson, Bruce C. ; Dolan, David F. ; Ptok, Martin U. ; Altschuler, Richard A. / Experimental model of immune-mediated hearing loss using cross-species immunization. In: Laryngoscope. 1990 ; Vol. 100, No. 9. pp. 941-947.
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