Experimental infection of human foreskin cultures with BK virus, a human papovavirus

K. V. Shah, C. Hudson, J. Valis, J. D. Strandberg

Research output: Contribution to journalArticle

Abstract

Cell cultures derived from foreskins of 19 newborn infants were exposed to irradiated or unirradiated BK virus (BKV), an SV40 related papovavirus infection of man, and were observed for viral cytopathic effect (CPE) and for possible transformation. Four of the cultures were passed serially and maintained for a period of 9 wk on medium containing BKV antiserum. All infected cultures developed viral CPE. Irradiation of virus delayed but did not prevent viral CPE. In nearly one half of the cultures, some regrowth occurred following extensive virus induced destruction of the cell sheets but the regrown cells, as a rule, remained infected with BKV and could not be passed. In 2 instances, regrown cells (JV 1 and JV 2) could be passed, and these had some of the characteristics of transformed cells. They were essentially free of BKV viral antigen but contained, in over 90% of the cells, the intranuclear T antigen. They were resistant to superinfection with BKV. In contrast to the uninfected control cells which were uniformly fibroblastic in appearance, cells in JV 1 and JV 2 cultures were pleomorphic with a predominance of stellate cells and multinucleated giant cells. However, JV 1 and JV 2 cultures were contact inhibited, did not reach high saturation densities, and could not be maintained beyond the 30th passage.

Original languageEnglish (US)
Pages (from-to)180-186
Number of pages7
JournalExperimental Biology and Medicine
Volume153
Issue number1
StatePublished - 1976

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BK Virus
Foreskin
Viruses
Cell culture
Viral Cytopathogenic Effect
Infection
Viral Antigens
Viral Tumor Antigens
Superinfection
Giant Cells
Immune Sera
Irradiation
Cell Culture Techniques
Newborn Infant

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Shah, K. V., Hudson, C., Valis, J., & Strandberg, J. D. (1976). Experimental infection of human foreskin cultures with BK virus, a human papovavirus. Experimental Biology and Medicine, 153(1), 180-186.

Experimental infection of human foreskin cultures with BK virus, a human papovavirus. / Shah, K. V.; Hudson, C.; Valis, J.; Strandberg, J. D.

In: Experimental Biology and Medicine, Vol. 153, No. 1, 1976, p. 180-186.

Research output: Contribution to journalArticle

Shah, KV, Hudson, C, Valis, J & Strandberg, JD 1976, 'Experimental infection of human foreskin cultures with BK virus, a human papovavirus', Experimental Biology and Medicine, vol. 153, no. 1, pp. 180-186.
Shah, K. V. ; Hudson, C. ; Valis, J. ; Strandberg, J. D. / Experimental infection of human foreskin cultures with BK virus, a human papovavirus. In: Experimental Biology and Medicine. 1976 ; Vol. 153, No. 1. pp. 180-186.
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