Abstract
A soluble lacrimal gland antigen (LG-Ag) capable of inducing experimental autoimmune dacryoadenitis (EAD) in SJL/J mice has been purified from bovine lacrimal gland extracts by a combination of ion exchange and gel-filtration chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed a single band with a mobility corresponding to a molecular weight of 45,000 daltons. Antibodies raised in guinea pigs against purified LG-Ag were used to confirm the tissue-specificity and to assess the cellular localization of LG-Ag within tissues. Anti-LG-Ag serum reacted with the crude lacrimal gland extracts but not with the extracts of bovine submaxillary gland, parotid gland, or retina. As shown by immunofluorescent staining, LG- Ag appeared to be localized on the murine lacrimal ductal epithelial cells but was lacking in the salivary ducts. LG-Ag induced EAD that was characterized by infiltration of mononuclear cells around the ducts and associated vasculature, accompanied by extensive damage of the acinar cells. The lesions induced were tissue-specific, in that SJL/J mice immunized with LG-Ag had intense lymphocytic infiltrates in lacrimal gland, but had no significant lesions in their salivary and harderian glands. The availability of a purified LG antigen can facilitate the further analysis of pathogenetic mechanisms in EAD.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2029-2036 |
| Number of pages | 8 |
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 33 |
| Issue number | 6 |
| State | Published - 1992 |
Keywords
- autoimmune dacryoadenitis
- dacryoadenitis
- lacrimal gland antigen
- lacrimal gland autoimmunity
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience