Experimental autoimmune dacryoadenitis: Purification and characterization of a lacrimal gland antigen

S. H. Liu, D. H. Zhou

Research output: Contribution to journalArticlepeer-review

Abstract

A soluble lacrimal gland antigen (LG-Ag) capable of inducing experimental autoimmune dacryoadenitis (EAD) in SJL/J mice has been purified from bovine lacrimal gland extracts by a combination of ion exchange and gel-filtration chromatography. Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed a single band with a mobility corresponding to a molecular weight of 45,000 daltons. Antibodies raised in guinea pigs against purified LG-Ag were used to confirm the tissue-specificity and to assess the cellular localization of LG-Ag within tissues. Anti-LG-Ag serum reacted with the crude lacrimal gland extracts but not with the extracts of bovine submaxillary gland, parotid gland, or retina. As shown by immunofluorescent staining, LG- Ag appeared to be localized on the murine lacrimal ductal epithelial cells but was lacking in the salivary ducts. LG-Ag induced EAD that was characterized by infiltration of mononuclear cells around the ducts and associated vasculature, accompanied by extensive damage of the acinar cells. The lesions induced were tissue-specific, in that SJL/J mice immunized with LG-Ag had intense lymphocytic infiltrates in lacrimal gland, but had no significant lesions in their salivary and harderian glands. The availability of a purified LG antigen can facilitate the further analysis of pathogenetic mechanisms in EAD.

Original languageEnglish (US)
Pages (from-to)2029-2036
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume33
Issue number6
StatePublished - 1992

Keywords

  • autoimmune dacryoadenitis
  • dacryoadenitis
  • lacrimal gland antigen
  • lacrimal gland autoimmunity

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Experimental autoimmune dacryoadenitis: Purification and characterization of a lacrimal gland antigen'. Together they form a unique fingerprint.

Cite this