Expansion of FasL-expressing CD5+ B cells in type 1 diabetes patients

Ankit Saxena, Hideo Yagita, Thomas W. Donner, Abdel Rahim A. Hamad

Research output: Research - peer-reviewArticle

Abstract

Fas ligand drives insulitis in the non-obese diabetic mouse model of type 1 diabetes (T1D) and negatively regulates IL-10-producing (IL-10pos) CD5+ B cells in pancreata. Relevance of these phenomena to the human disease is poorly understood. Here, using splenocytes from T1D, autoantibody (Ab+), and non-diabetic (ND) human subjects, we show that a subpopulation of CD5+ B cells that is characterized by expression of FasL (FasLhiCD5+) was significantly elevated in T1D subjects, many of whom had significantly reduced frequency of IL-10posCD5+ B cells compared to Ab+ subjects. The majority of FasLhiCD5+ B cells did not produce cytokines and were more highly resistant to activation-induced cell death than their IL-10posCD5+ counterparts. These results associate expansion of FasL-expressing CD5+ B cells with T1D and lay the groundwork for future mechanistic studies to understand specific role in disease pathogenesis.

LanguageEnglish (US)
Article number402
JournalFrontiers in Immunology
Volume8
Issue numberAPR
DOIs
StatePublished - Apr 7 2017

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Type 1 Diabetes Mellitus
B-Lymphocytes
Interleukin-10
Inbred NOD Mouse
Fas Ligand Protein
Autoantibodies
Pancreas
Cell Death
Cytokines

Keywords

  • Autoimmunity
  • B cell
  • CD5
  • Fas (CD95)
  • FasL (CD178)
  • Gld
  • IL-10
  • Type 1 diabetes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Expansion of FasL-expressing CD5+ B cells in type 1 diabetes patients. / Saxena, Ankit; Yagita, Hideo; Donner, Thomas W.; Hamad, Abdel Rahim A.

In: Frontiers in Immunology, Vol. 8, No. APR, 402, 07.04.2017.

Research output: Research - peer-reviewArticle

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