Expansion of antigen-specific T cells from cerebrospinal fluid of patients with multiple sclerosis

John R. Richert, Dale E. McFarlin, John W. Rose, Henry F. McFarland, Jeffrey I. Greenstein

Research output: Contribution to journalArticle

Abstract

We have expanded cerebrospinal fluid (CSF) T lymphocytes obtained from four patients with multiple sclerosis (MS). Fresh CSF cells were placed into culture with medium, interleukin-2, irradiated autologous peripheral blood mononuclear cells, and either myelin basic protein (BP) or measles virus antigen. Two CSF cell lines demonstrated a mild degree of antigen-specific proliferation to BP, a third reacted with measles virus, and the fourth demonstrated no known antigenic specificity. The percentage of HLA-DR + cells was increased in all four cell lines. The culture procedure has thus selected for a population of activated T cells, some of which demonstrate reactivity with antigens of potential relevance to the pathogenesis of MS.

Original languageEnglish (US)
Pages (from-to)317-324
Number of pages8
JournalJournal of Neuroimmunology
Volume5
Issue number3
DOIs
StatePublished - 1983
Externally publishedYes

Keywords

  • Antigenic specificity
  • Cerebrospinal fluid
  • Interleukin-2
  • Measles virus
  • Multiple sclerosis
  • T. lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

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