Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing

Wei Yan, Cory Smith, Linzhao Cheng

Research output: Contribution to journalArticle

Abstract

Our ability to precisely and efficiently edit mammalian and plant genomes has been significantly improved in recent years, partially due to increasing use of designer nucleases that recognize a pre-determined DNA sequence, make a specific DNA double-strand break, and stimulate gene targeting. A pair of zinc finger nucleases (ZFNs) or transcription activator-like effector nucleases (TALENs) that recognize two adjacent unique DNA sequences dimerize through the fused FokI nuclease domain and cut in the middle of target DNA sequences. We report here that increasing the length of recognition DNA sequences by TALENs or ZFNs does not necessarily translate to a higher efficiency or specificity. We also discover that one subunit of ZFNs and one subunit of TALENs can form a pair of hybrid nucleases with expanded specificity at two diverse targets, and stimulate gene targeting in multiple cell types including human induced pluripotent stem (iPS) cells with improved efficiency.

Original languageEnglish (US)
Article number2376
JournalScientific reports
Volume3
DOIs
StatePublished - Aug 19 2013

ASJC Scopus subject areas

  • General

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