Exon sequences distant from the splice junction are required for efficient self-splicing of the Tetrahymena IVS

Sarah A. Woodson

Research output: Contribution to journalArticle

Abstract

The presence of a natural rRNA secondary structure element immediately preceding the 5′ splice site of the Tetrahymena IVS can inhibit self-splicing by competing with base pairing between the 5′ exon and the guide sequence of the IVS (P1). Formation of this alternative hairpin is preferred in short precursor RNAs, and results in loss of G-addition to the 5′ splice site. Pre-rRNAs which contain longer exons of ribosomal sequence, however, splice rapidly. As many as 146 nucleotides of the 5′ exon and 86 nucleotides of the 3′ exon are required for efficient self-splicing of Tetrahymena precursors. The presence of nucleotides distant from the 5′ splice site apparently alters the equilibrium between the alternative hairpins, and promotes formation of active precursors. This effect is dependent on the specific sequences of the ribosomal pre-RNA, since point mutations within this region reduce the rate of splicing as much as 50-fold. This system provides an opportunity to study the way in which long-range interactions can influence splice site selection in a highly structured RNA.

Original languageEnglish (US)
Pages (from-to)4027-4032
Number of pages6
JournalNucleic Acids Research
Volume20
Issue number15
StatePublished - Aug 11 1992
Externally publishedYes

Fingerprint

Tetrahymena
Nucleotides
RNA
Precursor
RNA Splice Sites
Exons
RNA Precursors
Site selection
Long-range Interactions
Alternatives
Secondary Structure
Pairing
Ribosomal RNA
Immediately
Mutation
Fold
Point Mutation
Base Pairing
Dependent

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Exon sequences distant from the splice junction are required for efficient self-splicing of the Tetrahymena IVS. / Woodson, Sarah A.

In: Nucleic Acids Research, Vol. 20, No. 15, 11.08.1992, p. 4027-4032.

Research output: Contribution to journalArticle

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