TY - JOUR
T1 - Exon sequence distant from the splic junction are required fo0r efficient self-splicing of the Tetrahymena IVA
AU - Woodson, Sarah A.
N1 - Funding Information:
I wish to thank Cheryl Grosshans and Anne Ritter for synthesis of oligonucleotides, Arthur Zaug for the preparation of T7 RNA polymerase and gift of pJK43-T7 DNA, and Douglas Julin for critical reading of the manuscript. I also thank Tom Cech for his encouragement and generous support. This work was funded by an American Cancer Society Postdoctoral Fellowship to S.W., Howard Hughes Medical Institute (to T.R.C.), and the NIH (GM46686 to S.W.).
PY - 1992/8/11
Y1 - 1992/8/11
N2 - The presence of a natural rRNA secondary structure element immediately preceding the 5' splice site of the Tetrahymena IVS can Inhibit self-splicing by competing with base pairing between the 5' exon and the guide sequence of the IVS (P1). Formation of this alternative hairpin Is preferred in short precursor RNAs, and results in loss of G-addrtion to the 5' splice site. Pre-rRNAs which contain longer exons of ribosomal sequence, however, splice rapidly. As many as 146 nucleotides of the 5' exon and 86 nucleotides of the 3' exon are required for efficient self-splicing of Tetrahymena precursors. The presence of nucleotides distant from the 5' splice site apparently alters the equilibrium between the alternative hairpins, and promotes formation of active precursors. This effect is dependent on the specific sequences of the ribosomal pre-RNA, since point mutations within this region reduce the rate of splicing as much as 50-fold. This system provides an opportunity to study the way in which long-range interactions can influence splice site selection in a highly structured RNA.
AB - The presence of a natural rRNA secondary structure element immediately preceding the 5' splice site of the Tetrahymena IVS can Inhibit self-splicing by competing with base pairing between the 5' exon and the guide sequence of the IVS (P1). Formation of this alternative hairpin Is preferred in short precursor RNAs, and results in loss of G-addrtion to the 5' splice site. Pre-rRNAs which contain longer exons of ribosomal sequence, however, splice rapidly. As many as 146 nucleotides of the 5' exon and 86 nucleotides of the 3' exon are required for efficient self-splicing of Tetrahymena precursors. The presence of nucleotides distant from the 5' splice site apparently alters the equilibrium between the alternative hairpins, and promotes formation of active precursors. This effect is dependent on the specific sequences of the ribosomal pre-RNA, since point mutations within this region reduce the rate of splicing as much as 50-fold. This system provides an opportunity to study the way in which long-range interactions can influence splice site selection in a highly structured RNA.
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U2 - 10.1093/nar/20.15.4027
DO - 10.1093/nar/20.15.4027
M3 - Article
C2 - 1508687
AN - SCOPUS:0026776101
SN - 0305-1048
VL - 20
SP - 4027
EP - 4032
JO - Nucleic acids research
JF - Nucleic acids research
IS - 15
ER -