Exogenous specific fluorescence markers location reconstruction using surface fluorescence imaging

Avital Garashi, Israel Gannot, Victor Chernomordik, Gallya Gannot, Amir Gandjbakhche

Research output: Contribution to journalConference articlepeer-review

Abstract

Diseased tissue may be specifically marked by an exogenous fluorescent marker and then, following laser activation of the marker, optically and non-invasively detected through fluorescence imaging. Interaction of a fluorophore, conjugated to an appropriate antibody, with the antigen expressed by the diseased tissue, can indicate the presence of a specific disease. Using an optical detection system and a reconstruction algorithm, we were able to determine the fluorophore's position in the tissue. We present 3D reconstructions of the location of a fluorescent marker, FITC, in the tongues of mice. One group of BALB/c mice was injected with squamous cell carcinoma (SqCC) cell line to the tongue, while another group served as the control. After tumor development, the mice's tongues were injected with FITC conjugated to anti-CD3 and anti-CD19 antibodies. An Argon laser excited the marker at 488 nm while a high precision fluorescent camera collected the emitted fluorescence. Measurements were performed with the fluorescent marker embedded at various simulated depths. The simulation was performed using agarose-based gel slabs applied to the tongue as tissue-like phantoms. A biopsy was taken from every mouse after the procedure and the excised tissue was histologically evaluated. We reconstruct the fluorescent marker's location in 3D using an algorithm based on the random walk theory.

Original languageEnglish (US)
Pages (from-to)314-321
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4955
DOIs
StatePublished - Dec 5 2003
Externally publishedYes
EventPROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Tomography and Spectroscopy of Tissue V - San Jose, CA, United States
Duration: Jan 26 2003Jan 29 2003

Keywords

  • Agarose
  • Exogenous fluorophore
  • FITC
  • Fluorescence imaging
  • Optical diagnosis
  • Random walk theory

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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