Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor

Munekazu Yamakuchi; Marcella Ferlito; Craig N. Morrell; Kenji Matsushita; Craig A. Fletcher; Wangsen Cao; Charles J. Lowenstein

doi:10.1007/978-1-59745-178-9_15

Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor

Munekazu Yamakuchi, Marcella Ferlito, Craig N. Morrell, Kenji Matsushita, Craig A. Fletcher, Wangsen Cao, Charles J. Lowenstein

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Endothelial exocytosis of granules is a rapid response to vascular injury. However, the molecular machinery that regulates exocytosis in endothelial cells is not well understood. Recently developed techniques have defined the endothelial proteins that control vesicle and granule trafficking in endothelial cells. These techniques have revealed that syntaxin 4, synaptobrevin 3, and N-ethylmaleimide-sensitive factor (NSF) play a critical role in endothelial granule exocytosis. Additional studies have shown that nitric oxide regulates exocytosis by chemically modifying NSF. Further characterization of the factors that regulate exocytosis will lead to novel treatments for vascular diseases such as myocardial infarction and stroke.

Original language	English (US)
Title of host publication	Exocytosis and Endocytosis
Publisher	Humana Press
Pages	203-215
Number of pages	13
ISBN (Print)	9781588298652
DOIs	https://doi.org/10.1007/978-1-59745-178-9_15
State	Published - 2008

Publication series

Name	Methods in Molecular Biology
Volume	440
ISSN (Print)	1064-3745

Keywords

Granule
N-ethylmaleimide-sensitive factor
Nitric oxide
P-selectin
Vesicle
Von Willebrand factor
Weibel-Palade body

ASJC Scopus subject areas

Molecular Biology
Genetics

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Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor. / Yamakuchi, Munekazu; Ferlito, Marcella; Morrell, Craig N.; Matsushita, Kenji; Fletcher, Craig A.; Cao, Wangsen; Lowenstein, Charles J.

Exocytosis and Endocytosis. Humana Press, 2008. p. 203-215 (Methods in Molecular Biology; Vol. 440).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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