Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor.

Munekazu Yamakuchi, Marcella Ferlito, Craig N. Morrell, Kenji Matsushita, Craig A. Fletcher, Wangsen Cao, Charles J. Lowenstein

Research output: Contribution to journalArticle

Abstract

Endothelial exocytosis of granules is a rapid response to vascular injury. However, the molecular machinery that regulates exocytosis in endothelial cells is not well understood. Recently developed techniques have defined the endothelial proteins that control vesicle and granule trafficking in endothelial cells. These techniques have revealed that syntaxin 4, synaptobrevin 3, and N-ethylmaleimide-sensitive factor (NSF) play a critical role in endothelial granule exocytosis. Additional studies have shown that nitric oxide regulates exocytosis by chemically modifying NSF. Further characterization of the factors that regulate exocytosis will lead to novel treatments for vascular diseases such as myocardial infarction and stroke.

Original languageEnglish (US)
Pages (from-to)203-215
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume440
StatePublished - 2008

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N-Ethylmaleimide-Sensitive Proteins
Exocytosis
Endothelial Cells
Vesicle-Associated Membrane Protein 3
Qa-SNARE Proteins
Vascular System Injuries
Vascular Diseases
Nitric Oxide
Stroke
Myocardial Infarction
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yamakuchi, M., Ferlito, M., Morrell, C. N., Matsushita, K., Fletcher, C. A., Cao, W., & Lowenstein, C. J. (2008). Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor. Methods in molecular biology (Clifton, N.J.), 440, 203-215.

Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor. / Yamakuchi, Munekazu; Ferlito, Marcella; Morrell, Craig N.; Matsushita, Kenji; Fletcher, Craig A.; Cao, Wangsen; Lowenstein, Charles J.

In: Methods in molecular biology (Clifton, N.J.), Vol. 440, 2008, p. 203-215.

Research output: Contribution to journalArticle

Yamakuchi, M, Ferlito, M, Morrell, CN, Matsushita, K, Fletcher, CA, Cao, W & Lowenstein, CJ 2008, 'Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor.', Methods in molecular biology (Clifton, N.J.), vol. 440, pp. 203-215.
Yamakuchi M, Ferlito M, Morrell CN, Matsushita K, Fletcher CA, Cao W et al. Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor. Methods in molecular biology (Clifton, N.J.). 2008;440:203-215.
Yamakuchi, Munekazu ; Ferlito, Marcella ; Morrell, Craig N. ; Matsushita, Kenji ; Fletcher, Craig A. ; Cao, Wangsen ; Lowenstein, Charles J. / Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor. In: Methods in molecular biology (Clifton, N.J.). 2008 ; Vol. 440. pp. 203-215.
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