Excess in vitro prolactin secretion by pituitary cells from ovariectomized old rats.

M. Haji, G. S. Roth, Marc R. Blackman

Research output: Contribution to journalArticle

Abstract

Various in vivo and in vitro pituitary lactotropic and gonadotropic functions were measured in mature (6-7 mo, normally cycling) and old (24 mo, constant diestrus) female Wistar rats. Serum prolactin (PRL) levels were higher (P less than 0.001), whereas luteinizing hormone (LH) values were similar (P greater than 0.05) in old versus mature rats both before and 3 days after ovariectomy. Serum PRL levels decreased significantly (P less than 0.005) postovariectomy only in the mature rats. The in vitro release of PRL and LH was measured for 4 days in primary adenohypophyseal cell cultures from the ovariectomized rats. Both basal and 17 beta-estradiol (E2)-stimulated PRL release (P less than 0.001) and production (P less than 0.005) were greater by cells from old rats. In contrast, both basal release and E2-stimulated LH release were greater (P less than 0.001) by cells from mature rats. Peak PRL release by cells from both old and mature rats occurred after exposure to E2 doses 1/100th of those required for peak LH release. These data support the hypothesis that intrinsic derangements in anterior pituitary function contribute to the reproductive decline in aging female rats and that different pituitary cell types exhibit discordant age changes in estrogenic sensitivity.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume247
Issue number4 Pt 1
StatePublished - Oct 1984

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Lactotrophs
Prolactin
Luteinizing Hormone
Diestrus
Primary Cell Culture
Ovariectomy
In Vitro Techniques
Serum
Wistar Rats
Estradiol

ASJC Scopus subject areas

  • Medicine(all)

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Excess in vitro prolactin secretion by pituitary cells from ovariectomized old rats. / Haji, M.; Roth, G. S.; Blackman, Marc R.

In: The American journal of physiology, Vol. 247, No. 4 Pt 1, 10.1984.

Research output: Contribution to journalArticle

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