Examining the origins of the hydration force between lipid bilayers using all-atom simulations

Anastasia N. Gentilcore, Naveen Michaud-Agrawal, Paul S. Crozier, Mark J. Stevens, Thomas B Woolf

Research output: Contribution to journalArticle

Abstract

Using 237 all-atom double bilayer simulations, we examined the thermodynamic and structural changes that occur as a phosphatidylcholine lipid bilayer stack is dehydrated. The simulated system represents a micropatch of lipid multilayer systems that are studied experimentally using surface force apparatus, atomic force microscopy and osmotic pressure studies. In these experiments, the hydration level of the system is varied, changing the separation between the bilayers, in order to understand the forces that the bilayers feel as they are brought together. These studies have found a curious, strongly repulsive force when the bilayers are very close to each other, which has been termed the "hydration force," though the origins of this force are not clearly understood. We computationally reproduce this repulsive, relatively free energy change as bilayers come together and make qualitative conclusions as to the enthalpic and entropic origins of the free energy change. This analysis is supported by data showing structural changes in the waters, lipids and salts that have also been seen in experimental work. Increases in solvent ordering as the bilayers are dehydrated are found to be essential in causing the repulsion as the bilayers come together.

Original languageEnglish (US)
Pages (from-to)1-15
Number of pages15
JournalJournal of Membrane Biology
Volume235
Issue number1
DOIs
StatePublished - May 2010

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Lipid Bilayers
Lipids
Atomic Force Microscopy
Osmotic Pressure
Phosphatidylcholines
Thermodynamics
Salts
Water

Keywords

  • Entropy:enthalpy compensation
  • Hydration force
  • Molecular dynamics
  • Multilayer lipid system

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Cite this

Examining the origins of the hydration force between lipid bilayers using all-atom simulations. / Gentilcore, Anastasia N.; Michaud-Agrawal, Naveen; Crozier, Paul S.; Stevens, Mark J.; Woolf, Thomas B.

In: Journal of Membrane Biology, Vol. 235, No. 1, 05.2010, p. 1-15.

Research output: Contribution to journalArticle

Gentilcore, Anastasia N. ; Michaud-Agrawal, Naveen ; Crozier, Paul S. ; Stevens, Mark J. ; Woolf, Thomas B. / Examining the origins of the hydration force between lipid bilayers using all-atom simulations. In: Journal of Membrane Biology. 2010 ; Vol. 235, No. 1. pp. 1-15.
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