Examination of colonies and stool blots for detection of enteropathogens by DNA hybridization with eight DNA probes

P. Echeverria, D. N. Taylor, J. Seriwatana, J. E. Brown, U. Lexomboon

Research output: Contribution to journalArticle

Abstract

We compared three methods for detecting enteropathogens in 416 children with diarrhea: (i) examination of 10 lactose-fermenting and all non-lactose-fermenting Escherichia coli (colony blots); (ii) examination of 300 colonies (replicate blots); and (iii) determination of the total bacterial growth of stools (stool blots). All specimens were spotted onto Whatman 541 filters and hybridized with specific radiolabeled DNA probes. Enterotoxigenic E. coli was detected in 38 patients by examining colony blots, in 52 patients by examining replicate blots, and in 45 patients by examining stool blots. Enteropathogenic E. coli adhesion factor was detected in 12 patients by colony blots, in 25 patients by replicate blots, and in 16 patients by stool blots. E. coli that hybridized with the enterohemorrhagic E. coli probe was detected in 2 patients by colony blots, in 11 patients by replicate blots, and in 0 patients by stool blots. Shiga-like toxin-producing E. coli was detected in 0 patients by colony blots, in 12 patients by replicate blots, and in 0 patients by stool blots. Shigella spp. were identified by standard bacteriological methods in 82 patients, and enteroinvasive E. coli was identified by colony blots in 11 patients (total, 93), by replicate blots in 56 patients, and by stool blots in 35 patients. Of 82 culture-confirmed Shigella infections, 45 were identified by examining replicate blots with the 17-kilobase-pair probe and 36 were identified by examination with the Ipa probe (P <0.05). Examining replicate blots with specific probes identified more enterotoxigenic E. coli (P <0.05). Examining replicate blots with specific probes identified more enterotoxigenic E. coli (P <0.05), enteropathogenic E. coli adhesin factor-producing E. coli (P <0.001), and Shiga-like toxin-producing E. coli (P <0.005) infections than examining colony blots. More Shigella and enteroinvasive E. coli infections were identified by standard bacteriological methods and examining colony blots with a specific probe than by examining replicate and stool blots (P <0.001).

Original languageEnglish (US)
Pages (from-to)331-334
Number of pages4
JournalJournal of Clinical Microbiology
Volume27
Issue number2
StatePublished - 1989
Externally publishedYes

Fingerprint

DNA Probes
DNA
Escherichia coli
Enterotoxigenic Escherichia coli
Shigella
Shiga Toxins
Enteropathogenic Escherichia coli
Escherichia coli Adhesins
Enterohemorrhagic Escherichia coli
Escherichia coli Infections
Lactose
Infection

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Echeverria, P., Taylor, D. N., Seriwatana, J., Brown, J. E., & Lexomboon, U. (1989). Examination of colonies and stool blots for detection of enteropathogens by DNA hybridization with eight DNA probes. Journal of Clinical Microbiology, 27(2), 331-334.

Examination of colonies and stool blots for detection of enteropathogens by DNA hybridization with eight DNA probes. / Echeverria, P.; Taylor, D. N.; Seriwatana, J.; Brown, J. E.; Lexomboon, U.

In: Journal of Clinical Microbiology, Vol. 27, No. 2, 1989, p. 331-334.

Research output: Contribution to journalArticle

Echeverria, P, Taylor, DN, Seriwatana, J, Brown, JE & Lexomboon, U 1989, 'Examination of colonies and stool blots for detection of enteropathogens by DNA hybridization with eight DNA probes', Journal of Clinical Microbiology, vol. 27, no. 2, pp. 331-334.
Echeverria, P. ; Taylor, D. N. ; Seriwatana, J. ; Brown, J. E. ; Lexomboon, U. / Examination of colonies and stool blots for detection of enteropathogens by DNA hybridization with eight DNA probes. In: Journal of Clinical Microbiology. 1989 ; Vol. 27, No. 2. pp. 331-334.
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