Ex vivo soluble Fas ligand treatment of donor cells to selectively reduce murine acute graft versus host disease

Robert W. Georgantas, Osnat Bohana-Kashtan, Curt I. Civin

Research output: Contribution to journalArticle

Abstract

BACKGROUND. Allogeneic bone marrow transplantation (BMT) and donor lymphocyte infusion (DLI) provide valuable treatments for a range of diseases. However, the therapeutic utility of BMT and DLI is reduced by the high incidence of graft-versus-host disease (GvHD) mediated by activated donor T lymphocytes directed against recipient alloantigens. METHODS. Using mouse models, we developed and evaluated a strategy to selectively enhance activation-induced cell death (AICD) of anti-recipient T cells within transplant donor cell populations, with the goal of reducing GvHD. Responder T lymphocytes were incubated ex vivo with irradiated allogenic stimulator cells in a mixed lymphocyte reaction (MLR) in the presence of soluble Fas ligand (sFasL) to induce AICD in alloreactive cells. RESULTS. This ex vivo sFasL treatment reduced proliferation to the allogeneic stimulator cells in vitro and abrogated acute GvHD capacity in vivo. In contrast, the secondary immune responsiveness of the ex vivo sFasL-treated responder T cells to an unrelated model antigen was preserved. Furthermore, upon adoptive transfer in a DLI model, ex vivo sFasL-treated T cells were able to reject a model tumor. Finally, ex vivo sFasL treatment of bone marrow cells did not reduce their hematopoietic engraftment capacity. CONCLUSIONS. Thus, ex vivo treatment with sFasL appears to have potential for translation to clinical cell processing of BMT allografts and DLI infusions.

Original languageEnglish (US)
Pages (from-to)471-478
Number of pages8
JournalTransplantation
Volume82
Issue number4
DOIs
StatePublished - Aug 2006

Keywords

  • Bone marrow transplantation
  • Graft-versus-host disease (GVHD)
  • Tolerization

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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