Ex vivo normothermic hemoperfusion of the canine pancreas: Applications and limitations of a modified experimental preparation

Frederic Eckhauser, J. A. Knol, V. Porter-Fink, D. Lockery, Leslie Edgcomb, W. E. Strodel, David Webb, Jerry Simmons

Research output: Contribution to journalArticlepeer-review

Abstract

Ex vivo recirculation perfusion preparations of the canine pancreas are useful for the study of pancreatic physiology and exocrine secretory function. Vagaries of isolated ex vivo organ perfusion, however, require a thorough familiarity with available circulation media and component technology. Electrolyte and acid-base homeostasis can be facilitated by incorporating a dialysis unit into the perfusion circuit. Viability of the preparation is best assessed by constancy of vascular resistance and oxygen extraction during the period of perfusion. Limitations imposed by this closed method of organ perfusion on studies of pancreatic endocrine function and on duodenopancreatic hormonal interactions (enteroinsular axis) must be recognized.

Original languageEnglish (US)
Pages (from-to)22-37
Number of pages16
JournalJournal of Surgical Research
Volume31
Issue number1
DOIs
StatePublished - Jul 1981
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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