Ex vivo induction and expansion of natural killer T cells by CD1d1-Ig coated artificial antigen presenting cells

Tonya J. Webb, Joan G. Bieler, Jonathan P Schneck, Mathias Oelke

Research output: Contribution to journalArticle

Abstract

Natural killer T (NKT) cells play a pivotal role in maintaining immune homostasis. They recognize lipid antigen in the context of CD1d molecules and subsequently produce cytokines that activate cells of both the innate and adaptive immune responses. Many studies examining patients with autoimmune disease or cancer have shown that there is a reduction in both NKT cell number and function. Due to the complexities of manipulating NKT cells in vivo, ex vivo expanded effector NKT cells would be an excellent therapeutic modality. To date, immunotherapy utilizing the NKT/CD1d system has been dependent on the use of autologous DC in the presence or absence of a synthetic glycolipid, α-galactocylceramide. Here we report a novel technique that facilitates the growth and analysis of NKT cells through the use of CD1d-expressing aAPC. CD1d-based aAPC can effectively propagate both canonical (iNKT cells) and noncanonical (Vα14-) NKT cells. Importantly, CD1d-Ig aAPC can expand NKT cells from cancer patients. Thus, CD1d-expressing aAPC will enhance our knowledge of NKT cell biology and could potentially be used as a novel tool in adoptive immunotherapeutic strategies.

Original languageEnglish (US)
Pages (from-to)38-44
Number of pages7
JournalJournal of Immunological Methods
Volume346
Issue number1-2
DOIs
StatePublished - Jul 31 2009

Fingerprint

Natural Killer T-Cells
Antigen-Presenting Cells
CD1d Antigen
Glycolipids
Adaptive Immunity
Innate Immunity
Immunotherapy
Autoimmune Diseases
Cell Biology
Neoplasms
Cell Count
Cytokines
Lipids
Antigens

Keywords

  • aAPC
  • CD1d
  • NKT cells

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Ex vivo induction and expansion of natural killer T cells by CD1d1-Ig coated artificial antigen presenting cells. / Webb, Tonya J.; Bieler, Joan G.; Schneck, Jonathan P; Oelke, Mathias.

In: Journal of Immunological Methods, Vol. 346, No. 1-2, 31.07.2009, p. 38-44.

Research output: Contribution to journalArticle

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