TY - JOUR
T1 - Ex vivo graft purging and expansion of autologous blood progenitor cell products from patients with multiple myeloma
AU - Yang, Hong
AU - Robinson, Simon N.
AU - Nieto, Yago
AU - Jones, Richard J.
AU - Gocke, Christopher D.
AU - Lu, Junjun
AU - Giralt, Sergio A.
AU - Jones, Roy B.
AU - Decker, William K.
AU - Xing, Dongxia
AU - Steiner, David
AU - Champlin, Richard E.
AU - McMannis, John D.
AU - Ng, Jingjing
AU - Thomas, Michael W.
AU - Shah, Nina
AU - Andersson, Borje S.
AU - Parmar, Simrit
AU - Shpall, Elizabeth J.
PY - 2011/7/15
Y1 - 2011/7/15
N2 - Autologous peripheral blood progenitor cell (PBPC) transplantation is the treatment of choice for selected myeloma patients. However, tumor cells contaminating the apheresis product are a potential source of relapse. Here we report a sequential purging strategy targeting mature and immature clonogenic myeloma cell populations in the autograft. Thawed PBPC products of myeloma patients were treated with rituximab to kill CD138-20+ B cells (highly clonogenic immature cells), and bortezomib to target CD138 + cells (normal and differentiated myeloma plasma cells), followed by coculture with allogeneic mesenchymal stem cells (MSC) from normal donors. After 7 days of coculture, nonadherent cells were removed and cultured in the absence of MSC for an additional 7 days. Then, efficacy of purging (removal of CD138-20+ and CD138+ cells) was assessed by flow cytometry and PCR. We used our ex vivo purging strategy to treat frozen aphereses from 16 patients. CD138+ and CD138-20 +(19+) cells present in the initial products were depleted more than 3 and 4 logs, respectively based on 106 flow-acquisition events, and to levels below the limit of detection by PCR. In contrast, total nucleated cell (TNC), CD34+ cell, and colony-forming cell numbers were increased by approximately 12 to 20, 8-, and 23-fold, respectively. Overall, ex vivo treatment of apheresis products with rituximab, bortezomib, and coculture with normal donor MSC depleted mature and immature myeloma cells from clinical aphereses while expanding the normal hematopoietic progenitor cell compartment.
AB - Autologous peripheral blood progenitor cell (PBPC) transplantation is the treatment of choice for selected myeloma patients. However, tumor cells contaminating the apheresis product are a potential source of relapse. Here we report a sequential purging strategy targeting mature and immature clonogenic myeloma cell populations in the autograft. Thawed PBPC products of myeloma patients were treated with rituximab to kill CD138-20+ B cells (highly clonogenic immature cells), and bortezomib to target CD138 + cells (normal and differentiated myeloma plasma cells), followed by coculture with allogeneic mesenchymal stem cells (MSC) from normal donors. After 7 days of coculture, nonadherent cells were removed and cultured in the absence of MSC for an additional 7 days. Then, efficacy of purging (removal of CD138-20+ and CD138+ cells) was assessed by flow cytometry and PCR. We used our ex vivo purging strategy to treat frozen aphereses from 16 patients. CD138+ and CD138-20 +(19+) cells present in the initial products were depleted more than 3 and 4 logs, respectively based on 106 flow-acquisition events, and to levels below the limit of detection by PCR. In contrast, total nucleated cell (TNC), CD34+ cell, and colony-forming cell numbers were increased by approximately 12 to 20, 8-, and 23-fold, respectively. Overall, ex vivo treatment of apheresis products with rituximab, bortezomib, and coculture with normal donor MSC depleted mature and immature myeloma cells from clinical aphereses while expanding the normal hematopoietic progenitor cell compartment.
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U2 - 10.1158/0008-5472.CAN-11-0842
DO - 10.1158/0008-5472.CAN-11-0842
M3 - Article
C2 - 21646477
AN - SCOPUS:79960385474
SN - 0008-5472
VL - 71
SP - 5040
EP - 5049
JO - Cancer Research
JF - Cancer Research
IS - 14
ER -