Abstract
The ex vivo effects of macrophage colony-stimulating factor (M-CSF) on antifungal and antibacterial activities of human elutriated monocytes were studied. Cells were isolated prior to the initiation of therapy, on day 3 and at week 7, in six patients with an advanced malignancy receiving M-CSF in a phase I study. Superoxide anion production by monocytes in response to N-formyl methionyl leucyl phenylalanine was enhanced at day 3 of therapy (P = 0.011). In addition, at day 3, fungicidal activity against blastoconidia of Candida albicans was enhanced by M-CSF treatment (P = 0.026), whereas antifungal activity against hyphae of Aspergillus fumigatus was not significantly changed. Bactericidal activity against Staphylococcus aureus was increased at day 3 (P = 0.004). By Northern blot analysis, M-CSF does not upregulate the expression of components of the NADPH-oxidase, the multi-component enzyme system responsible for generation of superoxide radicals by monocytes. Instead, the predominant effect of M-CSF on circulating monocytes is probably a post-transcriptional effect. In conclusion, these findings suggest that administration of M-CSF to patients may enhance microbicidal activities and thus may provide a useful adjunct to conventional antimicrobial therapy.
Original language | English (US) |
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Pages (from-to) | 42-48 |
Number of pages | 7 |
Journal | Cytokine |
Volume | 8 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1996 |
Externally published | Yes |
Keywords
- Aspergillus
- Candida
- M-CSF
- Monocyte
- Staphylococcus
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Biochemistry
- Molecular Biology
- Hematology