Ex vivo effects of macrophage colony-stimulating factor on human monocyte activity against fungal and bacterial pathogens

Emmanuel Roilides, Caron A. Lyman, Susan D. Mertins, David J. Cole, David Venzon, Philip A. Pizzo, Stephen J. Chanock, Thomas J. Walsh

Research output: Contribution to journalArticlepeer-review

Abstract

The ex vivo effects of macrophage colony-stimulating factor (M-CSF) on antifungal and antibacterial activities of human elutriated monocytes were studied. Cells were isolated prior to the initiation of therapy, on day 3 and at week 7, in six patients with an advanced malignancy receiving M-CSF in a phase I study. Superoxide anion production by monocytes in response to N-formyl methionyl leucyl phenylalanine was enhanced at day 3 of therapy (P = 0.011). In addition, at day 3, fungicidal activity against blastoconidia of Candida albicans was enhanced by M-CSF treatment (P = 0.026), whereas antifungal activity against hyphae of Aspergillus fumigatus was not significantly changed. Bactericidal activity against Staphylococcus aureus was increased at day 3 (P = 0.004). By Northern blot analysis, M-CSF does not upregulate the expression of components of the NADPH-oxidase, the multi-component enzyme system responsible for generation of superoxide radicals by monocytes. Instead, the predominant effect of M-CSF on circulating monocytes is probably a post-transcriptional effect. In conclusion, these findings suggest that administration of M-CSF to patients may enhance microbicidal activities and thus may provide a useful adjunct to conventional antimicrobial therapy.

Original languageEnglish (US)
Pages (from-to)42-48
Number of pages7
JournalCytokine
Volume8
Issue number1
DOIs
StatePublished - Jan 1996

Keywords

  • Aspergillus
  • Candida
  • M-CSF
  • Monocyte
  • Staphylococcus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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