Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow

John P. Chute, Abha A. Saini, Dennis J. Chute, Mark R. Wells, William B. Clark, David M. Harlan, Jenny Park, Margaret K. Stull, Curt Civin, Thomas A. Davis

Research output: Contribution to journalArticle

Abstract

Adult human bone marrow (ABM) is an important source of hematopoietic stem cells for transplantation in the treatment of malignant and nonmalignant diseases. However, in contrast to the recent progress that has been achieved with umbilical cord blood, methods to expand ABM stem cells for therapeutic applications have been disappointing. In this study, we describe a novel culture method that uses human brain endothelial cells (HUBECs) and that supports the quantitative expansion of the most primitive measurable cell within the adult bone marrow compartment, the nonobese diabetic/ severe combined immunodeficient (NOD/ SCID) repopulating cell (SRC). Coculture of human ABM CD34+ cells with brain endothelial cells for 7 days supported a 5.4-fold increase in CD34+ cells, induced more than 95% of the CD34+CD38- subset to enter cell division, and produced progeny that engrafted NOD/SCID mice at significantly higher rates than fresh ABM CD34+ cells. Using a limiting dilution analysis, we found the frequency of SRCs within fresh ABM CD34+ cells to be 1 in 9.9 × 105 cells. Following HUBEC culture, the estimated frequency of SRCs increased to 1 in 2.4 × 105 cells. All mice that received transplants of HUBEC-cultured cells showed B-lymphoid and myeloid differentiation, indicating that a primitive hematopoietic cell was preserved during culture. Noncontact HUBEC cultures also maintained SRCs at a level comparable to contact HUBEC cultures, suggesting that cell-to-cell contact was not required. These data demonstrate that human brain endothelial cells possess a unique hematopoietic activity that increases the repopulating capacity of adult human bone marrow.

Original languageEnglish (US)
Pages (from-to)4433-4439
Number of pages7
JournalBlood
Volume100
Issue number13
DOIs
StatePublished - Dec 15 2002

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Endothelial cells
Cell culture
Brain
Bone
Endothelial Cells
Bone Marrow
Bone Marrow Cells
Stem cells
Cells
Cell Culture Techniques
Transplants
Dilution
Blood
Inbred NOD Mouse
SCID Mice
Hematopoietic Stem Cell Transplantation
Coculture Techniques
Fetal Blood
Cell Division
Cultured Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Chute, J. P., Saini, A. A., Chute, D. J., Wells, M. R., Clark, W. B., Harlan, D. M., ... Davis, T. A. (2002). Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow. Blood, 100(13), 4433-4439. https://doi.org/10.1182/blood-2002-04-1238

Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow. / Chute, John P.; Saini, Abha A.; Chute, Dennis J.; Wells, Mark R.; Clark, William B.; Harlan, David M.; Park, Jenny; Stull, Margaret K.; Civin, Curt; Davis, Thomas A.

In: Blood, Vol. 100, No. 13, 15.12.2002, p. 4433-4439.

Research output: Contribution to journalArticle

Chute, JP, Saini, AA, Chute, DJ, Wells, MR, Clark, WB, Harlan, DM, Park, J, Stull, MK, Civin, C & Davis, TA 2002, 'Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow', Blood, vol. 100, no. 13, pp. 4433-4439. https://doi.org/10.1182/blood-2002-04-1238
Chute, John P. ; Saini, Abha A. ; Chute, Dennis J. ; Wells, Mark R. ; Clark, William B. ; Harlan, David M. ; Park, Jenny ; Stull, Margaret K. ; Civin, Curt ; Davis, Thomas A. / Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow. In: Blood. 2002 ; Vol. 100, No. 13. pp. 4433-4439.
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