Evolutionarily conserved requirement for core binding factor beta in the assembly of the human immunodeficiency virus/simian immunodeficiency virus Vif-Cullin 5-RING E3 ubiquitin ligase

Xue Han, Weizi Liang, Deping Hua, Xiaohong Zhou, Juan Du, Sean L. Evans, Qimeng Gao, Hong Wang, Rachel Viqueira, Wei Wei, Wenyan Zhang, Xiao Fang Yu

Research output: Contribution to journalArticlepeer-review

Abstract

The human immunodeficiency virus type 1 (HIV-1)-encoded virion infectivity factor (Vif) is required to inactivate the host restriction factor APOBEC3 by engaging Cullin 5 (Cul5)-RING ubiquitin ligase (CRL5). Core binding factor beta (CBF-β) is a novel regulator of Vif-CRL5 function; as yet, its mechanism of regulation remains unclear. In the present study, we demonstrate that CBF-β promotion of Vif-CRL5 assembly is independent of its influence on Vif stability and is also a conserved feature of primate lentiviral Vif proteins. Furthermore, CBF-β is critical for the formation of the Vif-ElonginB/ElonginC-Cul5 core E3 ubiquitin ligase complex in vitro. CBF-β from diverse vertebrate species supported HIV-1 Vif function, indicating the conserved nature of Vif-CBF-β interfaces. Considering the importance of the interaction between Vif and CBF-β in viral CRL5 function, disrupting this interaction represents an attractive pharmacological intervention against HIV-1.

Original languageEnglish (US)
Pages (from-to)3320-3328
Number of pages9
JournalJournal of virology
Volume88
Issue number6
DOIs
StatePublished - Mar 2014

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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