TY - JOUR
T1 - Evolution of the scrambled germline gene encoding α-telomere binding protein in three hypotrichous ciliates
AU - Prescott, Jason D.
AU - DuBois, Michelle L.
AU - Prescott, David M.
N1 - Funding Information:
Acknowledgements. This work is supported by NIGMS grant GM56161 to DMP and by University of Colorado Cancer Center Student Research fellowships to JDP. The experiments described comply with the current laws of the USA.
PY - 1998
Y1 - 1998
N2 - The micronuclear genes encoding α-telomere-binding protein (αTP) in Oxytricha trifallax and Stylonychia mytilus contain multiple internal eliminated segments, or IESs, that divide the gene into multiple parts called macronuclear destined segments, or MDSs. The MDSs have become disordered, or scrambled, during evolution. The scrambled structures of the αTP genes in Oxytricha trifallax and S. mytilus have been compared with the previously published scrambled structure of the αTP gene in O. nova. The scrambled patterns of the αTP gene in the three species are similar but show significant differences. The micronuclear genes in O. nova and S. mytilus consist of 13 IESs and 14 MDSs, but the gene in O. trifallax is divided into three additional MDSs by the presence of three additional IESs, believed to have been inserted into the O. trifallax αTP gene after divergence of O. trifallax from the other two species. Corresponding IESs among the three species have shifted along the DNA during evolution, presumably by a mutational mechanism that changes the short repeat sequences that flank IESs. The IESs also have changed markedly in length by insertion and/or deletion of nucleotides. Comparison of the putative αTP amino acid sequences in the three species reveals three conserved and three non-conserved domains. The 5' nontranslated regions of the gene-sized molecules encoding αTP contain several conserved segments, and the 3' nontranscribed trailer contains one conserved segment.
AB - The micronuclear genes encoding α-telomere-binding protein (αTP) in Oxytricha trifallax and Stylonychia mytilus contain multiple internal eliminated segments, or IESs, that divide the gene into multiple parts called macronuclear destined segments, or MDSs. The MDSs have become disordered, or scrambled, during evolution. The scrambled structures of the αTP genes in Oxytricha trifallax and S. mytilus have been compared with the previously published scrambled structure of the αTP gene in O. nova. The scrambled patterns of the αTP gene in the three species are similar but show significant differences. The micronuclear genes in O. nova and S. mytilus consist of 13 IESs and 14 MDSs, but the gene in O. trifallax is divided into three additional MDSs by the presence of three additional IESs, believed to have been inserted into the O. trifallax αTP gene after divergence of O. trifallax from the other two species. Corresponding IESs among the three species have shifted along the DNA during evolution, presumably by a mutational mechanism that changes the short repeat sequences that flank IESs. The IESs also have changed markedly in length by insertion and/or deletion of nucleotides. Comparison of the putative αTP amino acid sequences in the three species reveals three conserved and three non-conserved domains. The 5' nontranslated regions of the gene-sized molecules encoding αTP contain several conserved segments, and the 3' nontranscribed trailer contains one conserved segment.
UR - http://www.scopus.com/inward/record.url?scp=0031775351&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031775351&partnerID=8YFLogxK
U2 - 10.1007/s004120050311
DO - 10.1007/s004120050311
M3 - Article
C2 - 9880762
AN - SCOPUS:0031775351
SN - 0009-5915
VL - 107
SP - 293
EP - 303
JO - Chromosoma
JF - Chromosoma
IS - 5
ER -