Evolution of the HIV-1 nef gene in HLA-B*57 Positive Elite Suppressors

Maria Salgado, Timothy P. Brennan, Karen A. O'Connell, Justin Bailey, Stuart Campbell Ray, Robert F Siliciano, Joel N Blankson

Research output: Contribution to journalArticle

Abstract

Elite controllers or suppressors (ES) are HIV-1 infected patients who maintain viral loads of <50 copies/ml without antiretroviral therapy. CD8+ T cells are thought to play a key role in the control of viral replication and exert selective pressure on gag and nef in HLA-B*57 positive ES. We previously showed evolution in the gag gene of ES which surprisingly was mostly due to synonymous mutations rather than non-synonymous mutation in targeted CTL epitopes. This finding could be the result of structural constraints on Gag, and we therefore examined the less conserved nef gene. We found slow evolution of nef in plasma virus in some ES. This evolution is mostly due to synonymous mutations and occurs at a rate similar to that seen in the gag gene in the same patients. The results provide further evidence of ongoing viral replication in ES and suggest that the nef and gag genes in these patients respond similarly to selective pressure from the host.

Original languageEnglish (US)
Article number94
JournalRetrovirology
Volume7
DOIs
StatePublished - Nov 8 2010

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nef Genes
gag Genes
HIV-1
Viral Load
Epitopes
Viruses
T-Lymphocytes
Mutation
HLA-B57 antigen
Silent Mutation
Therapeutics

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Evolution of the HIV-1 nef gene in HLA-B*57 Positive Elite Suppressors. / Salgado, Maria; Brennan, Timothy P.; O'Connell, Karen A.; Bailey, Justin; Ray, Stuart Campbell; Siliciano, Robert F; Blankson, Joel N.

In: Retrovirology, Vol. 7, 94, 08.11.2010.

Research output: Contribution to journalArticle

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