Evolution of chitin-binding proteins in invertebrates

Zhicheng Shen, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticle

Abstract

Analysis of a group of invertebrate proteins, including chitinases and peritrophic matrix proteins, reveals the presence of chitin-binding domains that share significant amino acid sequence similarity. The data suggest that these domains evolved from a common ancestor which may be a protein containing a single chitin-binding domain. The duplication and transposition of this chitin-binding domain may have contributed to the functional diversification of chitin-binding proteins. Sequence comparisons indicated that invertebrate and plant chitin binding domains do not share significant amino acid sequence similarity, suggesting that they are not coancestral. However, both the invertebrate and the plant chitin-binding domains are cysteine-rich and have several highly conserved aromatic residues. In plants, cysteines have been elucidated in maintaining protein folding and aromatic amino acids in interacting with saccharides [Wright HT, Sanddrasegaram G, Wright CS (1991) J Mol Evol 33:283-294]. It is likely that these residues perform similar functions in invertebrates. We propose that the invertebrate and the plant chitin-binding domains share similar mechanisms for folding and saccharide binding and that they evolved by convergent evolution. Furthermore, we propose that the disulfide bonds and aromatic residues are hallmarks for saccharide-binding proteins.

Original languageEnglish (US)
Pages (from-to)341-347
Number of pages7
JournalJournal of Molecular Evolution
Volume48
Issue number3
StatePublished - 1999
Externally publishedYes

Fingerprint

Chitin
chitin
Invertebrates
binding proteins
Carrier Proteins
invertebrate
invertebrates
protein
aromatic compounds
amino acid
carbohydrates
Cysteine
folding
cysteine
Amino Acid Sequence
amino acid sequences
Protein folding
peritrophic membrane
Amino Acids
convergent evolution

Keywords

  • Chitin binding
  • Chitinase
  • Peritrophic matrix

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics(clinical)
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Evolution of chitin-binding proteins in invertebrates. / Shen, Zhicheng; Jacobs-Lorena, Marcelo.

In: Journal of Molecular Evolution, Vol. 48, No. 3, 1999, p. 341-347.

Research output: Contribution to journalArticle

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