Evidence that the head of Kinesin is sufficient for force generation and motility in vitro

Joy T. Yang, William M. Saxton, Russell J. Stewart, Elizabeth C. Raff, Lawrence S B Goldstein

Research output: Contribution to journalArticle

Abstract

Kinesin is a mechanochemical protein that converts the chemical energy in adenosine triphosphate into mechanical force for movement of cellular components along microtubules. The regions of the kinesin molecule responsible for generating movement were determined by studying the heavy chain of Drosophila kinesin, and its truncated forms, expressed in Escherichia coli. The results demon-strate that (i) kinesin heavy chain alone, without the light chains and other eukaryotic factors, is able to induce microtubule movement in vitro, and (ii) a fragment likely to contain only the kinesin head is also capable of inducing microtubule motility. Thus, the amino-terminal 450 amino acids of kinesin contain all the basic elements needed to convert chemical energy into mechanical force.

Original languageEnglish (US)
Pages (from-to)42-47
Number of pages6
JournalScience
Volume249
Issue number4964
StatePublished - 1990
Externally publishedYes

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Kinesin
Microtubules
Drosophila
In Vitro Techniques
Adenosine Triphosphate
Escherichia coli
Light
Amino Acids
Proteins

ASJC Scopus subject areas

  • General

Cite this

Yang, J. T., Saxton, W. M., Stewart, R. J., Raff, E. C., & Goldstein, L. S. B. (1990). Evidence that the head of Kinesin is sufficient for force generation and motility in vitro. Science, 249(4964), 42-47.

Evidence that the head of Kinesin is sufficient for force generation and motility in vitro. / Yang, Joy T.; Saxton, William M.; Stewart, Russell J.; Raff, Elizabeth C.; Goldstein, Lawrence S B.

In: Science, Vol. 249, No. 4964, 1990, p. 42-47.

Research output: Contribution to journalArticle

Yang, JT, Saxton, WM, Stewart, RJ, Raff, EC & Goldstein, LSB 1990, 'Evidence that the head of Kinesin is sufficient for force generation and motility in vitro', Science, vol. 249, no. 4964, pp. 42-47.
Yang JT, Saxton WM, Stewart RJ, Raff EC, Goldstein LSB. Evidence that the head of Kinesin is sufficient for force generation and motility in vitro. Science. 1990;249(4964):42-47.
Yang, Joy T. ; Saxton, William M. ; Stewart, Russell J. ; Raff, Elizabeth C. ; Goldstein, Lawrence S B. / Evidence that the head of Kinesin is sufficient for force generation and motility in vitro. In: Science. 1990 ; Vol. 249, No. 4964. pp. 42-47.
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