Evidence that the F2-isoprostane, 8-epi-prostaglandin F2α, is formed in vivo

Jason D. Morrow, Tanya A. Minton, Kamal F. Badr, L. Jackson Roberts

Research output: Contribution to journalArticlepeer-review

156 Scopus citations


F2-isoprostanes are prostaglandin (PG)F2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One F2-isoprostane whose formation should be favored is 8-epi-PGF. 8-Epi-PGF has been shown to exert potent bioactivity but proof that it is formed in vivo is lacking. Evidence is now presented suggesting that 8-epi-PGF is, in fact, formed in vivo by demonstrating that an endogenous F2-isoprostane with a retention time on capillary GC identical with that of 8-epi-PGF co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabelled 8-epi-PGF.

Original languageEnglish (US)
Pages (from-to)244-248
Number of pages5
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Issue number2
StatePublished - Jan 3 1994
Externally publishedYes


  • Eicosanoid
  • Free radical
  • Isoprostane
  • Lipid peroxidation
  • Prostaglandin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology


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