Evidence that the F2-isoprostane, 8-epi-prostaglandin F2α, is formed in vivo

Jason D. Morrow; Tanya A. Minton; Kamal F. Badr; L. Jackson Roberts

doi:10.1016/0005-2760(94)90128-7

Evidence that the F2-isoprostane, 8-epi-prostaglandin F2α, is formed in vivo

Jason D. Morrow, Tanya A. Minton, Kamal F. Badr, L. Jackson Roberts

Research output: Contribution to journal › Article › peer-review

156 Scopus citations

Abstract

F₂-isoprostanes are prostaglandin (PG)F₂-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One F₂-isoprostane whose formation should be favored is 8-epi-PGF_2α. 8-Epi-PGF_2α has been shown to exert potent bioactivity but proof that it is formed in vivo is lacking. Evidence is now presented suggesting that 8-epi-PGF_2α is, in fact, formed in vivo by demonstrating that an endogenous F₂-isoprostane with a retention time on capillary GC identical with that of 8-epi-PGF_2α co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabelled 8-epi-PGF_2α.

Original language	English (US)
Pages (from-to)	244-248
Number of pages	5
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	1210
Issue number	2
DOIs	https://doi.org/10.1016/0005-2760(94)90128-7
State	Published - Jan 3 1994
Externally published	Yes

Keywords

Eicosanoid
Free radical
Isoprostane
Lipid peroxidation
Prostaglandin

ASJC Scopus subject areas

Biochemistry
Biophysics
Endocrinology

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10.1016/0005-2760(94)90128-7

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title = "Evidence that the F2-isoprostane, 8-epi-prostaglandin F2α, is formed in vivo",

abstract = "F2-isoprostanes are prostaglandin (PG)F2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One F2-isoprostane whose formation should be favored is 8-epi-PGF2α. 8-Epi-PGF2α has been shown to exert potent bioactivity but proof that it is formed in vivo is lacking. Evidence is now presented suggesting that 8-epi-PGF2α is, in fact, formed in vivo by demonstrating that an endogenous F2-isoprostane with a retention time on capillary GC identical with that of 8-epi-PGF2α co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabelled 8-epi-PGF2α.",

keywords = "Eicosanoid, Free radical, Isoprostane, Lipid peroxidation, Prostaglandin",

author = "Morrow, {Jason D.} and Minton, {Tanya A.} and Badr, {Kamal F.} and Roberts, {L. Jackson}",

year = "1994",

month = jan,

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doi = "10.1016/0005-2760(94)90128-7",

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TY - JOUR

T1 - Evidence that the F2-isoprostane, 8-epi-prostaglandin F2α, is formed in vivo

AU - Morrow, Jason D.

AU - Minton, Tanya A.

AU - Badr, Kamal F.

AU - Roberts, L. Jackson

PY - 1994/1/3

Y1 - 1994/1/3

N2 - F2-isoprostanes are prostaglandin (PG)F2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One F2-isoprostane whose formation should be favored is 8-epi-PGF2α. 8-Epi-PGF2α has been shown to exert potent bioactivity but proof that it is formed in vivo is lacking. Evidence is now presented suggesting that 8-epi-PGF2α is, in fact, formed in vivo by demonstrating that an endogenous F2-isoprostane with a retention time on capillary GC identical with that of 8-epi-PGF2α co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabelled 8-epi-PGF2α.

AB - F2-isoprostanes are prostaglandin (PG)F2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One F2-isoprostane whose formation should be favored is 8-epi-PGF2α. 8-Epi-PGF2α has been shown to exert potent bioactivity but proof that it is formed in vivo is lacking. Evidence is now presented suggesting that 8-epi-PGF2α is, in fact, formed in vivo by demonstrating that an endogenous F2-isoprostane with a retention time on capillary GC identical with that of 8-epi-PGF2α co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabelled 8-epi-PGF2α.

KW - Eicosanoid

KW - Free radical

KW - Isoprostane

KW - Lipid peroxidation

KW - Prostaglandin

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DO - 10.1016/0005-2760(94)90128-7

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AN - SCOPUS:0028176727

SN - 1388-1981

VL - 1210

SP - 244

EP - 248

JO - BBA - Specialised Section On Lipids and Related Subjects

JF - BBA - Specialised Section On Lipids and Related Subjects

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ER -

Evidence that the F2-isoprostane, 8-epi-prostaglandin F2α, is formed in vivo

Abstract

Keywords

ASJC Scopus subject areas

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