Abstract
F2-isoprostanes are prostaglandin (PG)F2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One F2-isoprostane whose formation should be favored is 8-epi-PGF2α. 8-Epi-PGF2α has been shown to exert potent bioactivity but proof that it is formed in vivo is lacking. Evidence is now presented suggesting that 8-epi-PGF2α is, in fact, formed in vivo by demonstrating that an endogenous F2-isoprostane with a retention time on capillary GC identical with that of 8-epi-PGF2α co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabelled 8-epi-PGF2α.
Original language | English (US) |
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Pages (from-to) | 244-248 |
Number of pages | 5 |
Journal | Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism |
Volume | 1210 |
Issue number | 2 |
DOIs | |
State | Published - Jan 3 1994 |
Externally published | Yes |
Keywords
- Eicosanoid
- Free radical
- Isoprostane
- Lipid peroxidation
- Prostaglandin
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Endocrinology