Evidence of gene amplification in tunicamycin-resistant chinese hamster ovary cells

Jane R. Scocca, Karin O. Hartog, Sharon S. Krag

Research output: Contribution to journalArticlepeer-review

Abstract

Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast tranferase gene, ALG7. The data presented suggest moderate amplification of the N-acetylglucosaminyl-1-phosphate transferase gene occurred in these cells, consistent with the previously observed chromosomal translocations and increased enzymatic activity. This is the first example of gene amplification as a mechanism for aberrations in N-linked glycosylation.

Original languageEnglish (US)
Pages (from-to)1063-1069
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume156
Issue number3
DOIs
StatePublished - Nov 15 1988

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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