Evidence of gene amplification in tunicamycin-resistant chinese hamster ovary cells

Jane R. Scocca; Karin O. Hartog; Sharon S. Krag

doi:10.1016/S0006-291X(88)80740-X

Evidence of gene amplification in tunicamycin-resistant chinese hamster ovary cells

Jane R. Scocca, Karin O. Hartog, Sharon S. Krag

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast tranferase gene, ALG7. The data presented suggest moderate amplification of the N-acetylglucosaminyl-1-phosphate transferase gene occurred in these cells, consistent with the previously observed chromosomal translocations and increased enzymatic activity. This is the first example of gene amplification as a mechanism for aberrations in N-linked glycosylation.

Original language	English (US)
Pages (from-to)	1063-1069
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	156
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(88)80740-X
State	Published - Nov 15 1988
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(88)80740-X

Cite this

@article{b259e19339f448aba9103b5bbb30d091,

title = "Evidence of gene amplification in tunicamycin-resistant chinese hamster ovary cells",

abstract = "Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast tranferase gene, ALG7. The data presented suggest moderate amplification of the N-acetylglucosaminyl-1-phosphate transferase gene occurred in these cells, consistent with the previously observed chromosomal translocations and increased enzymatic activity. This is the first example of gene amplification as a mechanism for aberrations in N-linked glycosylation.",

author = "Scocca, {Jane R.} and Hartog, {Karin O.} and Krag, {Sharon S.}",

note = "Funding Information: We would like to thank Dr. J.B. Douros (Natural Products Branch, Division of Cancer Treatment, NCI) Cancer for the gift of the tunicamycin and John J. Scocca, Ann Lawler, Patricia Gearhart, Michael Hauser, and Mark Chernaik for their help. This work was supported by Public Health Service grant GM36570. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1988",

month = nov,

day = "15",

doi = "10.1016/S0006-291X(88)80740-X",

language = "English (US)",

volume = "156",

pages = "1063--1069",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Evidence of gene amplification in tunicamycin-resistant chinese hamster ovary cells

AU - Scocca, Jane R.

AU - Hartog, Karin O.

AU - Krag, Sharon S.

N1 - Funding Information: We would like to thank Dr. J.B. Douros (Natural Products Branch, Division of Cancer Treatment, NCI) Cancer for the gift of the tunicamycin and John J. Scocca, Ann Lawler, Patricia Gearhart, Michael Hauser, and Mark Chernaik for their help. This work was supported by Public Health Service grant GM36570. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1988/11/15

Y1 - 1988/11/15

N2 - Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast tranferase gene, ALG7. The data presented suggest moderate amplification of the N-acetylglucosaminyl-1-phosphate transferase gene occurred in these cells, consistent with the previously observed chromosomal translocations and increased enzymatic activity. This is the first example of gene amplification as a mechanism for aberrations in N-linked glycosylation.

AB - Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast tranferase gene, ALG7. The data presented suggest moderate amplification of the N-acetylglucosaminyl-1-phosphate transferase gene occurred in these cells, consistent with the previously observed chromosomal translocations and increased enzymatic activity. This is the first example of gene amplification as a mechanism for aberrations in N-linked glycosylation.

UR - http://www.scopus.com/inward/record.url?scp=0023781651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023781651&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(88)80740-X

DO - 10.1016/S0006-291X(88)80740-X

M3 - Article

C2 - 2847724

AN - SCOPUS:0023781651

SN - 0006-291X

VL - 156

SP - 1063

EP - 1069

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Evidence of gene amplification in tunicamycin-resistant chinese hamster ovary cells

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this