The T-cell-based difference in responsiveness to MTg between good and poor responder mice was investigated by the use of congenic mouse strains, B10.BR(H-2k) and B10.D2(H-2d), and a T-cell adjuvant, poly A:U. Poly A:U, administered 3 hr after MTg, was effective as adjuvant only in good responder B10.BR mice; antibody titers (both mercaptoethanol sensitive and resistant) to MTg were equivalent to those obtained when MTg was given as an emulsion in IFA. The incorporation of poly A:U with IFA and MTg further raised the titers to the levels of those produced with MTg in CFA. Despite high antibody titers, treatment with MTg and poly A:U led only to transient thyroid infiltration. Thus, poly A:U activated MTg-helper T cells in good responders. In contrast, poly A:U was ineffective in promoting antibodies to MTg in poor responder B10.D2 mice. These data provide evidence for the presence of MTg-reactive T cells in good responder mice.
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