Evidence for thyroglobulin-reactive T cells in good responder mice

Patricio S. Esquivel; Yi Chi M. Kong; Noel R. Rose

doi:10.1016/0008-8749(78)90169-7

Evidence for thyroglobulin-reactive T cells in good responder mice

Patricio S. Esquivel, Yi Chi M. Kong, Noel R. Rose

School of Medicine

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The T-cell-based difference in responsiveness to MTg between good and poor responder mice was investigated by the use of congenic mouse strains, B10.BR(H-2^k) and B10.D2(H-2^d), and a T-cell adjuvant, poly A:U. Poly A:U, administered 3 hr after MTg, was effective as adjuvant only in good responder B10.BR mice; antibody titers (both mercaptoethanol sensitive and resistant) to MTg were equivalent to those obtained when MTg was given as an emulsion in IFA. The incorporation of poly A:U with IFA and MTg further raised the titers to the levels of those produced with MTg in CFA. Despite high antibody titers, treatment with MTg and poly A:U led only to transient thyroid infiltration. Thus, poly A:U activated MTg-helper T cells in good responders. In contrast, poly A:U was ineffective in promoting antibodies to MTg in poor responder B10.D2 mice. These data provide evidence for the presence of MTg-reactive T cells in good responder mice.

Original language	English (US)
Pages (from-to)	14-19
Number of pages	6
Journal	Cellular Immunology
Volume	37
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(78)90169-7
State	Published - Apr 1978

ASJC Scopus subject areas

Immunology

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title = "Evidence for thyroglobulin-reactive T cells in good responder mice",

abstract = "The T-cell-based difference in responsiveness to MTg between good and poor responder mice was investigated by the use of congenic mouse strains, B10.BR(H-2k) and B10.D2(H-2d), and a T-cell adjuvant, poly A:U. Poly A:U, administered 3 hr after MTg, was effective as adjuvant only in good responder B10.BR mice; antibody titers (both mercaptoethanol sensitive and resistant) to MTg were equivalent to those obtained when MTg was given as an emulsion in IFA. The incorporation of poly A:U with IFA and MTg further raised the titers to the levels of those produced with MTg in CFA. Despite high antibody titers, treatment with MTg and poly A:U led only to transient thyroid infiltration. Thus, poly A:U activated MTg-helper T cells in good responders. In contrast, poly A:U was ineffective in promoting antibodies to MTg in poor responder B10.D2 mice. These data provide evidence for the presence of MTg-reactive T cells in good responder mice.",

author = "Esquivel, {Patricio S.} and Kong, {Yi Chi M.} and Rose, {Noel R.}",

note = "Funding Information: 1 This work was supported by U.S.P.H.S. grants CA 16426 and CA 02357 and presented in part at the 1976 meeting of the American Association of Immunologists. 2 Recipient of Fogarty Fellowship from the National Institutes of Health; present address : Department of Experimental Medicine, Austral University, Valdivia, Chile. s Abbreviations used in this paper: MTg, mouse thyroglobulin; poly A : U, polyadenylic-polyuridylic acid complex ; CFA, complete Freund{\textquoteright}s adjuvant ; IFA, incomplete Freund{\textquoteright}s adjuvant; BGG, bovine gamma globulin.",

year = "1978",

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doi = "10.1016/0008-8749(78)90169-7",

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AU - Esquivel, Patricio S.

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AU - Rose, Noel R.

N1 - Funding Information: 1 This work was supported by U.S.P.H.S. grants CA 16426 and CA 02357 and presented in part at the 1976 meeting of the American Association of Immunologists. 2 Recipient of Fogarty Fellowship from the National Institutes of Health; present address : Department of Experimental Medicine, Austral University, Valdivia, Chile. s Abbreviations used in this paper: MTg, mouse thyroglobulin; poly A : U, polyadenylic-polyuridylic acid complex ; CFA, complete Freund’s adjuvant ; IFA, incomplete Freund’s adjuvant; BGG, bovine gamma globulin.

PY - 1978/4

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N2 - The T-cell-based difference in responsiveness to MTg between good and poor responder mice was investigated by the use of congenic mouse strains, B10.BR(H-2k) and B10.D2(H-2d), and a T-cell adjuvant, poly A:U. Poly A:U, administered 3 hr after MTg, was effective as adjuvant only in good responder B10.BR mice; antibody titers (both mercaptoethanol sensitive and resistant) to MTg were equivalent to those obtained when MTg was given as an emulsion in IFA. The incorporation of poly A:U with IFA and MTg further raised the titers to the levels of those produced with MTg in CFA. Despite high antibody titers, treatment with MTg and poly A:U led only to transient thyroid infiltration. Thus, poly A:U activated MTg-helper T cells in good responders. In contrast, poly A:U was ineffective in promoting antibodies to MTg in poor responder B10.D2 mice. These data provide evidence for the presence of MTg-reactive T cells in good responder mice.

AB - The T-cell-based difference in responsiveness to MTg between good and poor responder mice was investigated by the use of congenic mouse strains, B10.BR(H-2k) and B10.D2(H-2d), and a T-cell adjuvant, poly A:U. Poly A:U, administered 3 hr after MTg, was effective as adjuvant only in good responder B10.BR mice; antibody titers (both mercaptoethanol sensitive and resistant) to MTg were equivalent to those obtained when MTg was given as an emulsion in IFA. The incorporation of poly A:U with IFA and MTg further raised the titers to the levels of those produced with MTg in CFA. Despite high antibody titers, treatment with MTg and poly A:U led only to transient thyroid infiltration. Thus, poly A:U activated MTg-helper T cells in good responders. In contrast, poly A:U was ineffective in promoting antibodies to MTg in poor responder B10.D2 mice. These data provide evidence for the presence of MTg-reactive T cells in good responder mice.

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Evidence for thyroglobulin-reactive T cells in good responder mice

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