Evidence for simian immunodeficiency virus-specific igm and igg response in peripheral blood mononuclear cells of serum enzyme-linked immunosorbent assay-negative nonhuman primates

T. Jehuda-Cohen, J. D. Powell, F. Villinger, A. E. Mayne, K. W. Sell, A. A. Ansari

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro polyclonal activation of peripheral blood mononuclear cells (PBMCs) from 70% of the simian immunodeficiency virus (SIV) serum enzyme-linked, immunosorbent assay (ELISA)-negative sooty mangabeys leads to synthesis and release of low but significant and reproducible levels of S1V- reactive antibodies, as determined by ELISA and Western blot analysis. The predominant isotype of SIV-reactive antibodies in the pokeweed mitogen (PWM) supernatant fluids from serum ELISA-negative mangabeys is IgM, whereas the predominant isotype of SIV-reactive antibodies in seropositive mangabeys is IgG. Depletion of CD8+ cells led to a marked increase in the levels of SIV-reactive antibodies detected in supernatant fluids from PWM- induced cultures from the serum ELISA-negative mangabeys. No evidence for such SIV-reactive antibodies has been found, to date, in similar unfractionated or CD8+ T-cell-depleted PWM-induced PBMC cultures from uninfected macaques. Supernatant fluids from PWM cultures of PBMCs from a select group of serum ELISA-negative mangabeys, when concentrated five times, were shown to give a Western blot profile against SIV, similar to the profile seen with plasma from seropositive infected macaques and mangabeys. Evidence is presented to show that these serum ELISA-negative mangabeys are most likely latently infected with SIV. This evidence, which was obtained in samples from such ELISA-negative mangabeys, includes the detection of reverse transcriptase activity and the presence of SIV p27 in supernatant fluids of phytohemagglutinin-stimulated PBMCs in vitro. In addition, the data show the presence of CD8+ T cells that regulate SIV-specific Ig synthesis and show the detection of gag sequences by the polymerase chain reaction. Thus, the PWM assay described herein may provide a valuable additional tool for detection of lentivirus infection before or in the absence of seroconversion.

Original languageEnglish (US)
Pages (from-to)539-550
Number of pages12
JournalJournal of acquired immune deficiency syndromes
Volume7
Issue number6
StatePublished - Jun 1994
Externally publishedYes

Keywords

  • Latent infection
  • Nonhuman primates
  • Polyclonal activation
  • Simian immunodeficiency virus infection
  • Tolerance

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)

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