Evidence for selection of homogeneously staining regions in a human melanoma cell line

J. M. Trent, F. H. Thompson, C. Ludwig

Research output: Contribution to journalArticle

Abstract

Cytogenetic analysis of tumor cells from a human malignant melanoma was performed on both the primary tumor colony-forming cells and a cell line (HA-A) established subsequently from the clonogenic population. Chromosome-banding analysis demonstrated identical karyotypic alterations in both the tumor colony-forming cells and the HA-A cell line, documenting their origin from a common precursor. The most distinctive chromosome alterations shared between tumor colony-forming cells and the HA-A cell line were double-minute bodies (DMs) and a homogeneously staining region (HSR) on chromosome 7 at band p22. This represents the first observation of DMs or HSRs in cells from a human malignant melanoma. The frequency of HSR-bearing cells observed in the original tumor was 90% of all cells examined. In contrast, serial chromosome harvests at early passage of the HA-A cell line revealed positive selection for HSR-bearing cells with concomitant loss of DM-containing cells after increasing time in vitro. Following the ninth serial passage in vitro, HSRs were observed in 100% of cells with DMs no longer observed in the HA-A cell line. The finding of an HSR-bearing marker in the original tumor sample supports the view that HSRs are not an artifact of in vitro monolayer growth. However, our results demonstrate that the frequency of HSR-bearing cells within established cell lines may reflect in vitro selection and therefore not accurately reflect the frequency of this population in vivo.

Original languageEnglish (US)
Pages (from-to)233-237
Number of pages5
JournalCancer Research
Volume44
Issue number1
StatePublished - 1984
Externally publishedYes

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Melanoma
Staining and Labeling
Cell Line
Neoplasms
Chromosomes
Chromosome Banding
Serial Passage
Chromosomes, Human, Pair 7
Cytogenetic Analysis
Artifacts
Population
Observation
In Vitro Techniques
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Trent, J. M., Thompson, F. H., & Ludwig, C. (1984). Evidence for selection of homogeneously staining regions in a human melanoma cell line. Cancer Research, 44(1), 233-237.

Evidence for selection of homogeneously staining regions in a human melanoma cell line. / Trent, J. M.; Thompson, F. H.; Ludwig, C.

In: Cancer Research, Vol. 44, No. 1, 1984, p. 233-237.

Research output: Contribution to journalArticle

Trent, JM, Thompson, FH & Ludwig, C 1984, 'Evidence for selection of homogeneously staining regions in a human melanoma cell line', Cancer Research, vol. 44, no. 1, pp. 233-237.
Trent, J. M. ; Thompson, F. H. ; Ludwig, C. / Evidence for selection of homogeneously staining regions in a human melanoma cell line. In: Cancer Research. 1984 ; Vol. 44, No. 1. pp. 233-237.
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