Evidence for dopamine D-2 receptors on cholinergic interneurons in the rat caudate-putamen

Valina L. Dawson; Ted M. Dawson; Francis M. Filloux; James K. Wamsley

doi:10.1016/0024-3205(88)90492-4

Evidence for dopamine D-2 receptors on cholinergic interneurons in the rat caudate-putamen

Valina L. Dawson, Ted M. Dawson, Francis M. Filloux, James K. Wamsley

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Abstract

The aziridinium ion of ethylcholine (AF64A) is a neurotoxin that has demonstrated selectivity for cholinergic neurons. Unilateral stereotaxic injection of AF64A into the caudate-putamen of rats, resulted in a decrease in dopamine D-2 receptors as evidenced by a decrease in [³H]-sulpiride binding. Dopamine D-1 receptors, labeled with [³H]-SCH 23390, were unchanged. The efficacy of the lesion was demonstrated by the reduction of Na⁺-dependent high affinity choline uptake sites labeled with [³H]-hemicholinium-3. These data indicate that a population of D-2 receptors are postsynaptic on cholinergic interneurons within the striatum of rat brain.

Original language	English (US)
Pages (from-to)	1933-1939
Number of pages	7
Journal	Life Sciences
Volume	42
Issue number	20
DOIs	https://doi.org/10.1016/0024-3205(88)90492-4
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(88)90492-4

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title = "Evidence for dopamine D-2 receptors on cholinergic interneurons in the rat caudate-putamen",

abstract = "The aziridinium ion of ethylcholine (AF64A) is a neurotoxin that has demonstrated selectivity for cholinergic neurons. Unilateral stereotaxic injection of AF64A into the caudate-putamen of rats, resulted in a decrease in dopamine D-2 receptors as evidenced by a decrease in [3H]-sulpiride binding. Dopamine D-1 receptors, labeled with [3H]-SCH 23390, were unchanged. The efficacy of the lesion was demonstrated by the reduction of Na+-dependent high affinity choline uptake sites labeled with [3H]-hemicholinium-3. These data indicate that a population of D-2 receptors are postsynaptic on cholinergic interneurons within the striatum of rat brain.",

author = "Dawson, {Valina L.} and Dawson, {Ted M.} and Filloux, {Francis M.} and Wamsley, {James K.}",

note = "Funding Information: The authors wish to thank Jane Stout for her excellent secretarial assistance. This project was funded by grants from the Scottish Rite Foundation and the Public Health Service (NS-22033).",

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T1 - Evidence for dopamine D-2 receptors on cholinergic interneurons in the rat caudate-putamen

AU - Dawson, Valina L.

AU - Dawson, Ted M.

AU - Filloux, Francis M.

AU - Wamsley, James K.

N1 - Funding Information: The authors wish to thank Jane Stout for her excellent secretarial assistance. This project was funded by grants from the Scottish Rite Foundation and the Public Health Service (NS-22033).

PY - 1988

Y1 - 1988

N2 - The aziridinium ion of ethylcholine (AF64A) is a neurotoxin that has demonstrated selectivity for cholinergic neurons. Unilateral stereotaxic injection of AF64A into the caudate-putamen of rats, resulted in a decrease in dopamine D-2 receptors as evidenced by a decrease in [3H]-sulpiride binding. Dopamine D-1 receptors, labeled with [3H]-SCH 23390, were unchanged. The efficacy of the lesion was demonstrated by the reduction of Na+-dependent high affinity choline uptake sites labeled with [3H]-hemicholinium-3. These data indicate that a population of D-2 receptors are postsynaptic on cholinergic interneurons within the striatum of rat brain.

AB - The aziridinium ion of ethylcholine (AF64A) is a neurotoxin that has demonstrated selectivity for cholinergic neurons. Unilateral stereotaxic injection of AF64A into the caudate-putamen of rats, resulted in a decrease in dopamine D-2 receptors as evidenced by a decrease in [3H]-sulpiride binding. Dopamine D-1 receptors, labeled with [3H]-SCH 23390, were unchanged. The efficacy of the lesion was demonstrated by the reduction of Na+-dependent high affinity choline uptake sites labeled with [3H]-hemicholinium-3. These data indicate that a population of D-2 receptors are postsynaptic on cholinergic interneurons within the striatum of rat brain.

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VL - 42

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EP - 1939

JO - Life Sciences

JF - Life Sciences

IS - 20

ER -

Evidence for dopamine D-2 receptors on cholinergic interneurons in the rat caudate-putamen

Abstract

ASJC Scopus subject areas

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