Evidence for coexistence of GABA and dopamine in neurons of the rat olfactory bulb

C. M. Gall, Stewart H Hendry, K. B. Seroogy, E. G. Jones, J. W. Haycock

Research output: Contribution to journalArticle

Abstract

Immunoreactivities for γ-aminobutyric acid (GABA) and the dopamine-synthesizing enzyme tyrosine hydroxylase (TH) were localized ultrastructurally and colocalized at the light microscopic level in neurons of the rat main olfactory bulb. By means of a simulataneous indirect immunofluorescence technique, GABA and TH immunoreactivities were found to coexist in a large number of neurons in the glomerular and external plexiform layers. Virtually all the TH-immunoreactive periglomerular neurons also contained GABA immunoreactivity (GABA-I) while there was an additional number of GABA-immunoreactive periglomerular cells (27%) which did not contain TH immunoreactivity (TH-I). In contrast, the numerous tufted-type neurons in the glomerular and superficial external plexiform layers which contained TH-I did not contain GABA-I. In the external plexiform layer (EPL), 41% of the immunoreactive neurons contained GABA-I alone, 24% contained TH-I alone, and 35% contained both. EPL neurons containing GABA-I only or both GABA-I and TH-I never exhibited tufted cell morphological characteristics and were generally of the short-axon type. Electron microscopic examination of GABA-I and TH-I elements in the glomerular layer detected morphologically similar periglomerular perikarya and intraglomerular processes immunoreactive for each substance and other neurons and processes of the same type containing neither GABA-I or TH-I. These data indicate that the classical neurotransmitters GABA and dopamine coexist in large numbers of neurons in the rat main olfactory bulb including characteristic periglomerular cells and certain other local-circuit neuronal types.

Original languageEnglish (US)
Pages (from-to)307-318
Number of pages12
JournalJournal of Comparative Neurology
Volume266
Issue number3
StatePublished - 1987
Externally publishedYes

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Olfactory Bulb
Dopaminergic Neurons
Tyrosine 3-Monooxygenase
gamma-Aminobutyric Acid
Neurons
GABAergic Neurons
Dopamine
Aminobutyrates
Indirect Fluorescent Antibody Technique
Neurotransmitter Agents
Axons
Electrons
Light
Enzymes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Gall, C. M., Hendry, S. H., Seroogy, K. B., Jones, E. G., & Haycock, J. W. (1987). Evidence for coexistence of GABA and dopamine in neurons of the rat olfactory bulb. Journal of Comparative Neurology, 266(3), 307-318.

Evidence for coexistence of GABA and dopamine in neurons of the rat olfactory bulb. / Gall, C. M.; Hendry, Stewart H; Seroogy, K. B.; Jones, E. G.; Haycock, J. W.

In: Journal of Comparative Neurology, Vol. 266, No. 3, 1987, p. 307-318.

Research output: Contribution to journalArticle

Gall, CM, Hendry, SH, Seroogy, KB, Jones, EG & Haycock, JW 1987, 'Evidence for coexistence of GABA and dopamine in neurons of the rat olfactory bulb', Journal of Comparative Neurology, vol. 266, no. 3, pp. 307-318.
Gall, C. M. ; Hendry, Stewart H ; Seroogy, K. B. ; Jones, E. G. ; Haycock, J. W. / Evidence for coexistence of GABA and dopamine in neurons of the rat olfactory bulb. In: Journal of Comparative Neurology. 1987 ; Vol. 266, No. 3. pp. 307-318.
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