Evidence for budding of human immunodeficiency virus type 1 selectively from glycolipid-enriched membrane lipid rafts

Dzung H. Nguyen, James E K Hildreth

    Research output: Contribution to journalArticle

    Abstract

    A number of recent studies have demonstrated the significance of detergent-insoluble, glycolipid-enriched membrane domains or lipid rafts, especially in regard to activation and signaling in T lymphocytes. These domains can be viewed as floating rafts composed of sphingolipids and cholesterol which sequester glycosylphosphatidylinositol (GPI)-linked proteins, such as Thy-1 and CD59. CD45, a 200-kDa transmembrane phosphatase protein, is excluded from these domains. We have found that human immunodeficiency virus type 1 (HIV-1) particles produced by infected T-cell lines acquire the GPI-linked proteins Thy-1 and cD59, as well as the ganglioside GM1, which is known to partition preferentially into lipid rafts. In contrast, despite its high expression on the cell surface, CD45 was poorly incorporated into virus particles. Confocal fluorescence microscopy revealed that HIV-1 proteins colocalized with Thy-1, CD59, GM1, and a lipid raft- specific fluorescent lipid, DiIC16(3), in uropods of infected Jurkat cells. CD45 did not colocalize with HIV-1 proteins and was excluded from uropods. Dot immunoassay of Triton X-100-extracted membrane fractions revealed that HIV-1 p17 matrix protein and gp41 were present in the detergent-resistant fractions and that [3H]myristic acid-labeled HIV Gag showed a nine-to-one enrichment in lipid rafts. We propose a model for the budding of HIV virions through lipid rafts whereby host cell cholesterol, sphingolipids, and GPI- linked proteins within these domains are incorporated into the viral envelope, perhaps as a result of preferential sorting of HIV Gag to lipid rafts.

    Original languageEnglish (US)
    Pages (from-to)3264-3272
    Number of pages9
    JournalJournal of Virology
    Volume74
    Issue number7
    DOIs
    StatePublished - 2000

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    glycolipids
    Glycolipids
    Membrane Lipids
    Human immunodeficiency virus 1
    HIV-1
    GPI-Linked Proteins
    Lipids
    lipids
    Virion
    proteins
    Human Immunodeficiency Virus Proteins
    sphingolipids
    HIV
    virion
    detergents
    Detergents
    T-lymphocytes
    cholesterol
    Membrane Microdomains
    G(M1) Ganglioside

    ASJC Scopus subject areas

    • Immunology

    Cite this

    Evidence for budding of human immunodeficiency virus type 1 selectively from glycolipid-enriched membrane lipid rafts. / Nguyen, Dzung H.; Hildreth, James E K.

    In: Journal of Virology, Vol. 74, No. 7, 2000, p. 3264-3272.

    Research output: Contribution to journalArticle

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