Evidence against the existence of a membrane form of murine IL-1α

L. L. Minnich-Carruth, J. Suttles, S. B. Mizel

Research output: Contribution to journalArticle

Abstract

Previous studies have demonstrated that paraformaldehyde-treated macrophages possess IL-1α activity in a variety of bioassay systems. However, no definitive biochemical data in support of the membrane IL-1α concept has been reported. The purpose of the present study was to determine if the biologic activity associated with treated cells is due to a membrane form of IL-1α or alternatively, to the leakage of IL-1α. If the former case was true, then the exposed membrane IL-1α should bind anti-IL-1α antibodies or be cleaved by mild trypsin treatment. In both instances, IL-1α activity should be lost when measured in a subsequent IL-1 bioassay. Our results indicate that pulsing paraformaldehyde-treated normal or cell line macrophages with anti-IL-1α antibodies or treating the cells with trypsin did not affect the ability of the treated cells to function in a murine thymocyte proliferation assay. Furthermore, the standard short term treatment of cells with paraformaldehyde (15 min) did not prevent the leakage of IL-1α from the cells or the processing of the precursor forms of the protein. When cells were treated with paraformaldehyde for 2 h, they no longer released IL-1α or possessed thymocyte stimulatory activity. We also found that short term glutaraldehyde treatment of macrophages completely blocked the release of IL-1α from cells as well as the appearance of cell-associated IL-1α activity. Our results support the conclusion that the stimulatory activity of paraformaldehyde-treated macrophages is not due to a membrane form of IL-1α but is, in fact, due to the continuous release of IL-1α from the cells.

Original languageEnglish (US)
Pages (from-to)526-530
Number of pages5
JournalJournal of Immunology
Volume142
Issue number2
StatePublished - 1989
Externally publishedYes

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Interleukin-1
Membranes
Macrophages
Thymocytes
Biological Assay
Trypsin
Protein Precursors
Antibodies
Glutaral

ASJC Scopus subject areas

  • Immunology

Cite this

Minnich-Carruth, L. L., Suttles, J., & Mizel, S. B. (1989). Evidence against the existence of a membrane form of murine IL-1α. Journal of Immunology, 142(2), 526-530.

Evidence against the existence of a membrane form of murine IL-1α. / Minnich-Carruth, L. L.; Suttles, J.; Mizel, S. B.

In: Journal of Immunology, Vol. 142, No. 2, 1989, p. 526-530.

Research output: Contribution to journalArticle

Minnich-Carruth, LL, Suttles, J & Mizel, SB 1989, 'Evidence against the existence of a membrane form of murine IL-1α', Journal of Immunology, vol. 142, no. 2, pp. 526-530.
Minnich-Carruth LL, Suttles J, Mizel SB. Evidence against the existence of a membrane form of murine IL-1α. Journal of Immunology. 1989;142(2):526-530.
Minnich-Carruth, L. L. ; Suttles, J. ; Mizel, S. B. / Evidence against the existence of a membrane form of murine IL-1α. In: Journal of Immunology. 1989 ; Vol. 142, No. 2. pp. 526-530.
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