Evidence against a role for interleukin-10 in the regulation of growth of Mycobacterium avium in human monocytes

Hiroe Shiratsuchi, Beverly Hamilton, Zahra Toossi, Jerrold J. Ellner

Research output: Contribution to journalArticlepeer-review

Abstract

Interleukin-10 (IL-10) inhibits intracellular Mycobacterium avium killing by cytokine-activated murine macrophages and may have a role in pathogenesis. Cytokine activities in supernatants of M. avium-infected human monocytes were maximal at 6-24 h for tumor necrosis factor (TNF)-α and 24-48 h for IL-10. TNF-α and IL-10 production increased with increasing M. avium-to-monocyte infection ratios (20:1 to 200:1). TNF-α production by monocytes infected with smooth, domed, and opaque organisms at 200:1 exceeded that of monocytes infected with smooth, flat, and transparent M. avium (P <.01). IL-10 induction demonstrated considerable strain-to-strain variability and did not correlate with intracellular M. avium growth. IL-10 significantly inhibited TNF-α, IL-1β, and IL-6 production by M. avium-infected monocytes. Coculturing monocytes with IL-10 after M. avium infection did not affect intracellular M. avium growth. Differential induction of TNF-α may be a factor in the intracellular growth of M. avium in human monocytes. IL-10, however, played no apparent rule in pathogenicity in this model.

Original languageEnglish (US)
Pages (from-to)410-417
Number of pages8
JournalJournal of Infectious Diseases
Volume173
Issue number2
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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