Evaluation of Win 49,596, a novel steroidal androgen receptor antagonist, in animal models of prostate cancer

P. E. Juniewicz, N. Fetrow, J. Marinelli, M. Wolf, E. Young, J. Lamb, J. T. Isaacs

Research output: Contribution to journalArticle

Abstract

A series of experiments were conducted to evaluate the effects of Win 49,596, a novel steroidal androgen receptor antagonist, in animal models of prostate cancer. In the first experiment, oral administration of Win 49,596 at doses of 30, 100, or 300 mg/kg/day for 28 days inhibited (P <0.05) the growth of the androgen‐sensitive PAP variant of the Dunning R‐3327 prostatic carcinoma in intact male rats relative to intact controls. The degree of inhibition at 100 and 300 mg/kg/day Win 49,596 was similar (P >0.10) to that observed in castrate controls as well as in intact rats administered the nonsteroidal androgen receptor antagonist flutamide orally at 15 mg/kg/day. Castration as well as treatment with either Win 49,596 or flutamide also decreased (P <0.05) the weight of the prostate in tumor‐bearing animals. Additional studies were conducted to determine the effect of Win 49,596 on the growth of the androgen‐dependent PC‐82 human prostatic carcinoma xenografted into athymic nude male mice. Oral administration of Win 49,596 at 30, 100, or 300 mg/kg/day for 35 days inhibited (P <0.05) tumor growth relative to intact controls. The degree of tumor inhibition was similar to that observed in intact male mice administered the nonsteroidal androgen receptor antagonist flutamide orally at 30 mg/kg/day but was less than that observed following castration. Ventral prostate weights were also reduced (P <0.05) in castrate mice as well as in intact mice administered either Win 49,596 or flutamide. In the last experiment, at equivalent total daily dosages of either 150 or 300 mg/kg/day Win 49,596, twice a day (BID) dosing was more effective than once a day (SID) dosing in inhibiting tumor growth. The inhibitory effects of Win 49,596 at 150 mg/kg BID on tumor growth were similar to those observed following castration. Although Win 49,596 treatment reduced (P <0.05) ventral prostate weights relative to intact controls, there was no difference (P >0.10) between SID vs. BID dosing. Based on the results of these studies and subject to further testing, Win 49,596 may have utility in the treatment of hormonally dependent metastatic prostate cancer in humans.

Original languageEnglish (US)
Pages (from-to)105-115
Number of pages11
JournalThe Prostate
Volume18
Issue number2
DOIs
StatePublished - 1991

Keywords

  • Dunning R‐3327
  • PC‐82
  • flutamide
  • prostatic carcinoma

ASJC Scopus subject areas

  • Oncology
  • Urology

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