Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters

Otto D. Simmons, Mark D. Sobsey, Frank W. Schaefer, Donna S. Francy, Rebecca A. Nally, Christopher David Heaney

Research output: Contribution to journalArticle

Abstract

To improve surveillance for Cryptosporidium oocysts in water, the US Environmental Protection Agency developed method 1622, which consists of filtration, concentration, immunomagnetic separation, fluorescent antibody and 4, 6-diamidino-2-phenylindole (DAPI) counter-staining, and microscopic evaluation. Two filters were compared for analysis of 11 stream water samples collected throughout the United States. Replicate 10-L stream water samples (unspiked and spiked with 100-250 oocysts) were tested to evaluate matrix effects. Oocyst recoveries from the stream water samples averaged 22% (standard deviation [SD] = ±17%) with a membrane disk and 12% (SD = ±6%) with a capsule filter. Oocyst recoveries from reagent water precision and recovery samples averaged 39% (SD = ±13%) with a membrane disk and 47% (SD = ±19%) with a capsule filter. These results demonstrate that Cryptosporidium oocysts can be recovered from stream waters using method 1622, but recoveries are lower than those from reagent-grade water. This research also evaluated concentrations of indicator bacteria in the stream water samples. Because few samples were oocyst-positive, relationships between detections of oocysts and concentrations of indicator organisms could not be determined.

Original languageEnglish (US)
Pages (from-to)78-87
Number of pages10
JournalJournal / American Water Works Association
Volume93
Issue number1
StatePublished - Jan 2001
Externally publishedYes

Fingerprint

Water
Recovery
water
filter
Capsules
membrane
Membranes
method
detection
evaluation
Environmental Protection Agency
Antibodies
antibody
Bacteria
bacterium
matrix
indicator

ASJC Scopus subject areas

  • Civil and Structural Engineering
  • Water Science and Technology

Cite this

Simmons, O. D., Sobsey, M. D., Schaefer, F. W., Francy, D. S., Nally, R. A., & Heaney, C. D. (2001). Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters. Journal / American Water Works Association, 93(1), 78-87.

Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters. / Simmons, Otto D.; Sobsey, Mark D.; Schaefer, Frank W.; Francy, Donna S.; Nally, Rebecca A.; Heaney, Christopher David.

In: Journal / American Water Works Association, Vol. 93, No. 1, 01.2001, p. 78-87.

Research output: Contribution to journalArticle

Simmons, OD, Sobsey, MD, Schaefer, FW, Francy, DS, Nally, RA & Heaney, CD 2001, 'Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters', Journal / American Water Works Association, vol. 93, no. 1, pp. 78-87.
Simmons, Otto D. ; Sobsey, Mark D. ; Schaefer, Frank W. ; Francy, Donna S. ; Nally, Rebecca A. ; Heaney, Christopher David. / Evaluation of USEPA method 1622 for detection of Cryptosporidium oocysts in stream waters. In: Journal / American Water Works Association. 2001 ; Vol. 93, No. 1. pp. 78-87.
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