Evaluation of the spindle apparatus of in-vitro matured human oocytes following cryopreservation

S. G. Baka, T. L. Toth, L. L. Veeck, H. W. Jones, S. J. Muasher, S. E. Lanzendorf

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149 Scopus citations


The present study was conducted to determine if the cryopreservation of immature human oocytes has a deleterious effect on the meiotic spindle following maturation in vitro. Oocytes were obtained in excess from in-vitro fertilization patients and divided into four groups. Groups 1 (n = 98) and 2 (n = 80) consisted of immature oocytes cryopreserved before or after maturation in vitro respectively. Groups 3 (n = 37) and 4 (n = 9) served as non-frozen controls and included oocytes matured in vitro and in vivo respectively. The meiotic spindle was identified after incubation in anti-tubulin monoclonal antibody (1 h, 37°C) and fluorescein-conjugated goat anti-mouse immunoglobulin G (IgG) (1 h, RT). Chromosomes were counterstained with 4', 6'-diamidino-2-phenylindole. Following cryopreservation, group 1 oocytes demonstrated a 63% survival rate and 68% maturation rate in vitro. In all, 58% of the oocytes in group 2 survived the thaw. The number of oocytes with normal spindles in group 1 (81.0%) was not significantly different from control groups 3 (83.8%) and 4 (88.9%), while the number of group 2 oocytes with normal structures (43.5%) was significantly lower than groups 1 (P = 0.0004), 3 (P = 0.0002), and 4 (P = 0.025). These results suggest that cryopreservation of the prophase I human oocyte does not significantly increase abnormalities in the resulting meiotic spindle.

Original languageEnglish (US)
Pages (from-to)1816-1820
Number of pages5
JournalHuman Reproduction
Issue number7
StatePublished - Jul 1995


  • Cryopreservation
  • Human oocyte
  • IVF
  • Meiotic spindle

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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